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Target antigen
RFC1
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
mouse, rat
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Analyses
WB,IHC-P
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Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human RFC1(46-64aa NSSRKEDDFKQKQPSKKKR).
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the RFC1 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The RFC1 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
RFC(Replication Factor C), also called activator-1, is a multimeric primer-recognition protein consisting of 5 distinct subunits of 145, 40, 38, 37, and 36.5 kD. Human RFC was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40(SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.
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Related articles
1. Bermudez, V. P., Maniwa, Y., Tappin, I., Ozato, K., Yokomori, K., Hurwitz, J. The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA. Proc. Nat. Acad. Sci. 100: 10237-10242, 2003. 2. Peoples, R., Perez-Jurado, L., Wang, Y.-K., Kaplan, P., Francke, U. The gene for replication factor C subunit 2 (RFC2) is within the 7q11.23 Williams syndrome deletion. (Letter)Am. J. Hum. Genet. 58: 1370-1373, 1996.
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Gene Name
RFC1
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Protein Name
Replication factor C subunit 1
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Gene Full Name
replication factor C(activator 1) 1, 145kDa
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Synonyms
A1 140 kDa subunit antibody|A1 antibody|A1 P145 Activator 1 large subunit antibody|Activator 1 140 kDa subunit antibody|Activator 1 large subunit antibody|Activator 1 subunit 1 antibody|DNA binding Protein PO GA antibody|DNA-binding protein PO-GA antibody|MHC binding factor beta antibody|MHCBFB antibody|PO GA antibody|RECC1 antibody|Replication factor C(activator 1) 1, 145kDa antibody|Replication factor C 140 kDa subunit antibody|Replication factor C antibody|Replication factor C large subunit antibody|Replication factor C subunit 1 antibody|Replication factor C1 antibody|RF-C 140 kDa subunit antibody|RFC antibody|RFC1 antibody|RFC1_HUMAN antibody|RFC140 antibody|RFC140 Replication Factor C 140 kDa subunit antibody
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Uniprot ID
P35251
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
