REA Antibody

• Catalog number
  PA1951
• Price
  Please ask
• Size
  0,1 mg

• Target antigen
  REA
• Clonality
  Polyclonal antibody
• Clone
  Polyclonal antibody
• Raised in
  rabbit
• Type of the antibody
  IgG polyclonal antibody
• Product form
  freeze-dried
• Reacts with species
  human Predicted to work with: mouse, rat
• Analyses
  WB,IHC-P,ICC
• Immunogen
  A synthetic peptide corresponding to a sequence in the middle region of human REA(111-128aa RPNAQELPSMYQRLGLDY), identical to the related rat and mouse sequences.
• Product configuration
  Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Purification
  Immunogen affinity purified.
• Solubilization
  The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
• Storage condtions
  Keep the REA Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
• Tips
  The REA Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
• Background
  PHB2(Prohibitin 2), also called Repressor of Estrogen Receptor Activity(REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al.(1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta(ESR2), REA suppressed activation of a reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity.
• Related articles
  1. Merkwirth, C., Dargazanli, S., Tatsuta, T., Geimer, S., Lower, B., Wunderlich, F. T., von Kleist-Retzow, J.-C., Waisman, A., Westermann, B., Langer, T. Prohibitins control cell proliferation and apoptosis by regulating OPA1-dependent cristae morphogenesis in mitochondria. Genes Dev. 22: 476-488, 2008. 2. Montano, M. M., Ekena, K., Delage-Mourroux, R., Chang, W., Martini, P., Katzenellenbogen, B. S. An estrogen receptor-selective coregulator that potentiates the effectiveness of antiestrogens and represses the activity of estrogens. Proc. Nat. Acad. Sci. 96: 6947-6952, 1999. 3. Mussi, P., Liao, L., Park, S.-E., Ciana, P., Maggi, A., Katzenellenbogen, B. S., Xu, J., O'Malley, B. W. Haploinsufficiency of the corepressor of estrogen receptor activity (REA) enhances estrogen receptor function in the mammary gland. Proc. Nat. Acad. Sci. 103: 16716-16721, 2006.
• Gene Name
  PHB2
• Protein Name
  Prohibitin-2
• Gene Full Name
  prohibitin 2
• Synonyms
  B cell receptor associated protein BAP37 antibody|B-cell associated protein antibody|B-cell receptor-associated protein BAP37 antibody|Bap 37 antibody|BAP antibody|Bap37 antibody|BCAP 37 antibody|BCAP37 antibody|D prohibitin antibody|D-prohibitin antibody|MGC117268 antibody|p22 antibody|PHB 2 antibody|PHB2 antibody|PHB2_HUMAN antibody|PNAS 141 antibody|PNAS141 antibody|Prohibitin 2 antibody|Prohibitin-2 antibody|Prohibitin2 antibody|Repressor of Estrogen Receptor Activity antibody
• Uniprot ID
  Q99623
• Properties
  If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  REA  
• Gene symbol
  PHB2
• Short name
  REA Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Alternative name
  REA (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:30306
• Gene
  PHB2
• Long gene name
  prohibitin 2
• Synonyms
  • REA
  • BCAP37
  • Bap37
  • p22
• Synonyms name
  • B cell receptor associated protein 37
• GenBank acession
  • AF126021
• Locus
  12p13.31
• Discovery year
  2005-02-24
• Entrez gene record
  11331
• Pubmed identfication
  • 11302691
  • 9259555
• RefSeq identity
  • NM_007273
• VEGA ID
  OTTHUMG00000168519

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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