Nephrin Antibody

• Catalog number
  PB9113
• Price
  Please ask
• Size
  0,1 mg

• Target antigen
  Nephrin
• Clonality
  Polyclonal antibody
• Clone
  Polyclonal antibody
• Raised in
  rabbit
• Type of the antibody
  IgG polyclonal antibody
• Product form
  freeze-dried
• Reacts with species
  human
• Analyses
  WB,IHC-P,IHC-F
• Immunogen
  E.coli-derived human Nephrin recombinant protein (Position: Q23-P383). Human Nephrin shares 79% and 80% amino acid (aa) sequences identity with mouse and rat Nephrin, respectively.
• Product configuration
  Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Purification
  Immunogen affinity purified.
• Solubilization
  The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
• Storage condtions
  Keep the Nephrin Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
• Tips
  The Nephrin Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
• Background
  NPHS1, also called Nephrin, it is mapped to 19q13.12. This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. A defect in the gene for nephrin, NPHS1, is associated with congenital nephrotic syndrome of the Finnish type and causes massive amounts of protein to be leaked into the urine, orproteinuria. NPHS1 is also required for cardiovascular development.
• Related articles
  1. Wagner, N; Morrison, H; Pagnotta, S; Michiels, JF; Schwab, Y; Tryggvason, K; Schedl, A; Wagner, KD (2011-03-29). "The podocyte protein nephrin is required for cardiac vessel formation.". Human Molecular Genetics 20 (11): 2182–94. 2. Wartiovaara, J., Ofverstedt, L.-G., Khoshnoodi, J., Zhang, J., Makela, E., Sandin, S., Ruotsalainen, V., Cheng, R. H., Jalanko, H., Skoglund, U., Tryggvason, K. Nephrin strands contribute to a porous slit diaphragm scaffold as revealed by electron tomography. J. Clin. Invest. 114: 1475-1483, 2004.
• Gene Name
  NPHS1
• Protein Name
  Nephrin
• Gene Full Name
  nephrosis 1, congenital, Finnish type (nephrin)
• Synonyms
  CNF antibody|Nephrin antibody|Nephrosis 1 congenital Finnish type antibody|Nephrosis 1, congenital, Finnish type (nephrin) antibody|NPHN antibody|NPHN_HUMAN antibody|NPHS 1 antibody|NPHS1 antibody|Renal glomerulus specific cell adhesion receptor antibody|Renal glomerulus-specific cell adhesion receptor antibody
• Uniprot ID
  O60500
• Entrez GeneID
  4868
• Properties
  If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Nephrin  
• Gene symbol
  NPHS1, KIRREL1, KIRREL3
• Short name
  Nephrin Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Alternative name
  Nephrin (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:7908
• Gene
  NPHS1
• Long gene name
  NPHS1 adhesion molecule, nephrin
• Synonyms gene name
  • nephrosis 1, congenital, Finnish type (nephrin)
  • NPHS1, nephrin
• Synonyms
  • CNF
  • NPHN
• Locus
  19q13.12
• Discovery year
  1994-12-14
• Entrez gene record
  4868
• Pubmed identfication
  • 9915943
  • 9660941
• Classification
  • Fibronectin type III domain containing
  • C2-set domain containing
  • V-set domain containing
• VEGA ID
  OTTHUMG00000180689
• Locus Specific Databases
  • LRG_693

Gene info

• Identity
  HGNC:15734
• Gene
  KIRREL1
• Long gene name
  kirre like nephrin family adhesion molecule 1
• Synonyms gene
  • KIRREL
• Synonyms gene name
  • kin of IRRE like (Drosophila)
• Synonyms
  • NEPH1
• Synonyms name
  • nephrin-like protein 1
• GenBank acession
  • AK001707
• Locus
  1q23.1
• Discovery year
  2002-01-22
• Entrez gene record
  55243
• Pubmed identfication
  • 12424224
• RefSeq identity
  • NM_018240
• Classification
  • C2-set domain containing
  • I-set domain containing
• VEGA ID
  OTTHUMG00000022438

Gene info

• Identity
  HGNC:23204
• Gene
  KIRREL3
• Long gene name
  kirre like nephrin family adhesion molecule 3
• Synonyms gene name
  • kin of IRRE like 3 (Drosophila)
• Synonyms
  • NEPH2
  • KIAA1867
  • KIRRE
• GenBank acession
  • AB058770
• Locus
  11q24.2
• Discovery year
  2003-10-09
• Entrez gene record
  84623
• Pubmed identfication
  • 12424224
  • 11347906
• RefSeq identity
  • NM_032531
• Classification
  • C2-set domain containing
  • I-set domain containing
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000165831

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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