Mouse Legumain(total) PicoKine™ ELISA Kit
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Catalog numberEK1567
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Price:
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Size1x96 well plate
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ELISA s applicationFor quantitative measurement of mouse Legumain total in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
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Gene testedmurine Legumain(total)
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ELISA standard curveConcentration(pg/ml) + 0 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 + 2000 O.D. + 0.014 + 0.136 + 0.185 + 0.299 + 0.505 + 0.863 + 1.570 + 2.304
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ELISA kit detection limits31.2pg/ml to 2000pg/ml
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ELISA s sensitivity< 10pg/ml
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Target of this ELISANatural and recombinant mouse Legumain(total)
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Cross reactionthe ELISA does not cross react with any other proteins except from closely related species
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ELISA s precision parametersintra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);45;157;679;51;230;857 ELISA standard deviations 2.39;10.83;45.50;3.16;13.58;35.14 CV(%);5.3;6.9;6.7;6.2;5.9;4.1
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ELISA kit descriptionGENTAUR's mouse Legumain(total) ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for Legumain(total) was used as capure antibodies on the ELISA test plate. The internal standards (Expression system for standard: NSO, Immunogen sequence: V18-Y435) and your samples are measured with a biotin conjugated polyclonal from goat specific for Legumain(total) will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse Legumain(total) in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
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Detector and capure antibodiesA rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
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The ELISA containsCatalog number;Description;Quantity -;96-well plate precoated with anti- mouse Legumain(total) antibodies;1 ST0000-10;Lyophilized recombinant mouse Legumain(total) standard;10ng/tube×2 AR1107;Biotinylated anti- mouse Legumain(total) antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
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Anti coagulationheparin or EDTA
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How the elisa worksSample the amount of 100ul/well of the standard solution at 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse Legumain(total) standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, cell lysate, tissue homogenates, serum or plasma (heparin, EDTA) to the ELISA plate. Keep in mind that each mouse Legumain(total) standard solution and each sample be measured in duplicate.
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Minutes of TMB incubation20-25
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More gene infoLegumain (asparaginyl endopeptidase, citvac, proteinase B, hemoglobinase, PRSC1 gene product or LGMN (Homo sapiens), vicilin peptidohydrolase, bean endopeptidase) is an enzyme that in humans is encoded by the LGMN gene (previous symbolPRSC1). Using fluorescence in situ hybridization, the LGMN gene was mapped to chromosome 14q32.1. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types.
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AntigenNSO,V18-Y435
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Keep atStore at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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UniprotLegumain
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Gene namelegumain
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AlternativeAEP ; Asparaginyl endopeptidase ; cysteine 1 ; Cysteine protease 1 ; EC 3.4.22.34 ; Legumain ; LGMN ; LGMN_HUMAN ; LGMN1 ; Protease ; Protease cysteine 1 ; Protease cysteine 1 (legumain) ; PRSC1 ; Q99538
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Gen protein identityO89017
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Entrez nr19141
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolLgmn
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Short nameMouse Legumain( ) PicoKine™ ELISA Kit
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TechniqueELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
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Hostmouse, antigen assay ELISA kit 1 Kit (96 Wells)
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SpeciesMouses
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Alternative nameMouse Legumain(total) PicoKine™ Enzyme-linked immunosorbent assay reagent
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Alternative techniquemurine, elisas, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissuetotal
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Gene info
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Identity
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Gene
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Long gene namelegumain
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Synonyms gene
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Synonyms gene name
- protease, cysteine, 1 (legumain)
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-12-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data