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Target antigen
MEK7
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, mouse, rat
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Analyses
WB,IHC-P
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Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MEK7 (2-40aa AASSLEQKLSRLEAKLKQENREARRRIDLNLDISPQRPR), identical to the related mouse and rat sequences.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the MEK7 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The MEK7 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
MAP2K7(Mitogen-activated protein kinase kinase 7), also known as MAP kinase kinase 7, MAPKK7, JNKK2, PRKMK7 or MKK7, is an enzyme that in humans is encoded by the MAP2K7 gene. This protein is a member of the mitogen-activated protein kinase kinase family. The MKK7 protein exists as six different isoforms with three possible N-termini (α, β, and γ isoforms) and two possible C-termini (1 and 2 isoforms). Schramek et al. (2011) showed that the doxorubicin-mediated DNA damage response in human A549 lung carcinoma cells caused rapid phosphorylation and upregulation of p53 (TP53). MKK7 knockdown reduced p53 phosphorylation, delayed p53 upregulation, and interfered with cell cycle arrest at G2/M. MKK7 was activated in primary lung tumors, and tumors with a p53 mutation showed even higher MKK7 phosphorylation. Schramek et al. (2011) concluded that MKK7 exerts its tumor suppressive function through p53.
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Related articles
1. Lu, X., Nemoto, S., Lin, A. Identification of c-jun NH(2)-terminal protein kinase (JNK)-activating kinase 2 as an activator of JNK but not p38. J. Biol. Chem. 272: 24751-24754, 1997. 2. Schramek, D., Kotsinas, A., Meixner, A., Wada, T., Elling, U., Pospisilik, J. A., Neely, G. G., Zwick, R.-H., Sigl, V., Forni, G., Serrano, M., Gorgoulis, V. G., Penninger, J. M. The stress kinase MKK7 couples oncogenic stress to p53 stability and tumor suppression. Nature Genet. 43: 212-219, 2011. 3. Wu, Z., Wu, J., Jacinto, E., Karin, M. Molecular cloning and characterization of human JNKK2, a novel jun NH(2)-terminal kinase-specific kinase. Molec. Cell. Biol. 17: 7407-7416, 1997.
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Gene Name
MAP2K7
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Protein Name
Dual specificity mitogen-activated protein kinase kinase 7
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Gene Full Name
mitogen-activated protein kinase kinase 7
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Synonyms
c Jun N terminal kinase kinase 2 antibody|c-Jun N-terminal kinase kinase 2 antibody|Dual specificity mitogen activated protein kinase kinase 7 antibody|Dual specificity mitogen-activated protein kinase kinase 7 antibody|JNK activating kinase 2 antibody|JNK kinase 2 antibody|JNK-activating kinase 2 antibody|JNKK 2 antibody|Jnkk-2 antibody|Jnkk2 antibody| MAP kinase kinase 7 antibody|MAP2K 7 antibody|MAP2K7 antibody|MAPK/ERK kinase 7 antibody|MAPKK 7 antibody|MAPKK-7 antibody|MAPKK7 antibody|MEK 7 antibody|Mitogen activated protein kinase kinase 7 antibody|MKK 7 antibody|MKK-7 antibody|MKK7 antibody|MP2K7_HUMAN antibody|PRKMK 7 antibody|PRKMK-7 antibody| PRKMK7 antibody|Protein kinase mitogen activated kinase 7 antibody|Sek 2 antibody|Sek-2 antibody|Sek2 antibody|SKK4 antibody
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Uniprot ID
O14733
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Entrez GeneID
5609
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
