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Target antigen
MEK6
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, rat Predicted to work with: mouse
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MEK6(7-23aa KKRNPGLKIPKEAFEQP), identical to the related mouse sequence and different from the related rat sequence by one amino acid.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the MEK6 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The MEK6 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
MAP2K6(Mitogen-activated protein kinase kinase 6), also known as MAP kinase kinase 6(MAPKK 6) or MAPK/ERK kinase 6 is an enzyme that in humans is encoded by the MAP2K6 gene, on chromosome 17. MAPKK 6 is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein(MAP) kinase kinase. Han et al.(1996) described experiments addressing the function of MKK6 in intracellular signaling pathways leading toward activation of p38 MAPK. Mukherjee et al.(2006) found that the Yersinia YopJ virulence factor acted as an acetyltransferase, using coenzyme A to modify critical ser and thr residues in the MAP2K6 activation loop, thereby blocking phosphorylation and kinase activation. They proposed that ser and/or thr acetylation by a bacterial effector may be a mechanism that occurs in other signaling pathways.
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Related articles
1. Han, J., Lee, J.-D., Jiang, Y., Li, Z., Feng, L., Ulevitch, R. J. Characterization of the structure and function of a novel MAP kinase kinase (MKK6). J. Biol. Chem. 271: 2886-2891, 1996. 2. Mao, Z., Bonni, A., Xia, F., Nadal-Vicans, M., Greenberg, M. E. Neuronal activity-dependent cell survival mediated by transcription factor MEF2. Science 286: 785-790, 1999. 3. Mukherjee, S., Keitany, G., Li, Y., Wang, Y., Ball, H. L., Goldsmith, E. J., Orth, K. Yersinia YopJ acetylates and inhibits kinase activation by blocking phosphorylation. Science 312: 1211-1214, 2006.
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Gene Name
MAP2K6
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Protein Name
Dual specificity mitogen-activated protein kinase kinase 6
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Gene Full Name
mitogen-activated protein kinase kinase 6
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Synonyms
Dual specificity mitogen activated protein kinase kinase 6 antibody|Dual specificity mitogen-activated protein kinase kinase 6 antibody|EC 2.7.12.2 antibody|MAP kinase kinase 6 antibody|MAP2K6 antibody|MAPK/ERK kinase 6 antibody|MAPKK 6 antibody|MAPKK6 antibody|MEK 6 antibody|MEK6 antibody|Mitogen Activated Protein Kinase Kinase 6 antibody|MKK 6 antibody|MKK6 antibody|MP2K6_HUMAN antibody|PRKMK6 antibody|Protein kinase mitogen activated kinase 6 antibody|protein kinase, mitogen-activated, kinase 6(MAP kinase kinase 6) antibody|SAPK kinase 3 antibody|SAPKK-3 antibody|SAPKK3 antibody|SKK3 antibody|Stress-activated protein kinase kinase 3 antibody
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Uniprot ID
P52564
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
