Mast Cell Tryptase Antibody

• Catalog number
  PB10016
• Price
  Please ask
• Size
  0,1 mg

• Target antigen
  Mast Cell Tryptase
• Clonality
  Polyclonal antibody
• Clone
  Polyclonal antibody
• Raised in
  rabbit
• Type of the antibody
  IgG polyclonal antibody
• Product form
  freeze-dried
• Reacts with species
  human
• Analyses
  WB,IHC-P,ELISA
• Immunogen
  E. coli-derived human Mast Cell Tryptase recombinant protein (Position: H65-P275). Human Mast Cell Tryptase shares 77% and 76.1% amino acid (aa) sequence identity with mouse and rat Mast Cell Tryptase, respectively.
• Product configuration
  Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Purification
  Immunogen affinity purified.
• Solubilization
  The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
• Storage condtions
  Keep the Mast Cell Tryptase Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
• Tips
  The Mast Cell Tryptase Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
• Background
  Tryptase alpha-1 and tryptase beta-1 are enzymes that in humans are encoded by the same TPSAB1 gene. Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. In addition, these genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
• Related articles
  1. "Entrez Gene: TPSAB1 tryptase alpha/beta 1". 2. Miller JS, Moxley G, Schwartz LB (Oct 1990). "Cloning and characterization of a second complementary DNA for human tryptase". J Clin Invest 86 (3): 864–70. 3. Pallaoro M, Fejzo MS, Shayesteh L, Blount JL, Caughey GH (Feb 1999). "Characterization of genes encoding known and novel human mast cell tryptases on chromosome 16p13.3". J Biol Chem 274 (6): 3355–62.
• Gene Name
  TPSAB1
• Protein Name
  Tryptase alpha/beta-1
• Gene Full Name
  tryptase alpha/beta 1
• Synonyms
  alpha II | Lung tryptase | MCP 7 | Pituitary tryptase | Skin tryptase | TPS 1 | TPS1 | TPS2 | TPSAB1 | TPSAB1 protein | TPSB1 | Tryptase 1 | Tryptase alpha 1 | tryptase alpha I included | Tryptase alpha II | tryptase alpha II included | tryptase alpha included | tryptase alpha/beta 1 | Tryptase beta 1 | tryptase beta I included | Tryptase I | tryptase I included | Tryptase III | Tryptase skin | Q15661
• Uniprot ID
  Q15661
• Entrez GeneID
  7177
• Properties
  If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Description
  For cells, cell lines and tissues in culture till half confluency.
• French translation
  anticorps

• Gene target
  Mast   Tryptase  
• Short name
  Mast Tryptase Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Alternative name
  Mast cellular Tryptase (antibody to-)
• Alternative technique
  antibodies
• Tissue
  cell

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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