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ELISA s application
For quantitative measurement of human Tissue factor in cell culture supernatant, serum, plasma heparin, EDTA, citrate , Cell lysate, and urine.
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Gene tested
Human Tissue factor
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ELISA standard curve
Concentration(pg/ml) + 0 + 15.6 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 O.D. + 0.035 + 0.090 + 0.155 + 0.254 + 0.479 + 0.914 + 1.446 + 2.308
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ELISA kit detection limits
15.6pg/ml to 1000pg/ml
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ELISA s sensitivity
< 10pg/ml
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Target of this ELISA
Natural and recombinant human Tissue factor
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Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
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ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);106;285;593;162;321;630 ELISA standard deviations 3.71;15.39;29.06;9.07;22.15;36.54 CV(%);3.5;5.4;4.9;5.6;6.9;5.8
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ELISA kit description
GENTAUR's human Tissue factor ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for Tissue factor was used as capure antibodies on the ELISA test plate. The internal standards (NSO, S33-E251) and your samples are measured with a biotin conjugated polyclonal from goat specific for Tissue factor will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human Tissue factor in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
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Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
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The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human Tissue factor antibodies;1 ST0000-10;Lyophilized recombinant human Tissue factor standard;10ng/tube×2 AR1107;Biotinylated anti- human Tissue factor antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
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Anti coagulation
heparin, EDTA or citrate
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How the elisa works
Sample the amount of 100ul/well of the standard solution at 1000 pg per ml, 500, 25, 125, 62.5, 31.25, 15.6 pg/ml human Tissue factor standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum, plasma(heparin, EDTA, citrate) cell lysate or urine to the ELISA plate. Keep in mind that each human Tissue factor standard solution and each sample be measured in duplicate.
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Minutes of TMB incubation
15-20
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More gene info
Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. An incorrect synonym is thromboplastin. the F3 gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.
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Antigen
NSO, S33-E251
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Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Uniprot
Tissue factor
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Gene name
coagulation factor III (thromboplastin, tissue factor)
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Alternative
CD142;Coagulation factor III;F3;TF;TF_HUMAN;TFA;Thromboplastin;Tissue factor
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Gen protein identity
P13726
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Entrez nr
2152
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. 6
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Gene target
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Gene symbol
F3
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Short name
Tissue factor/F3 PicoKine ELISA Kit
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Technique
ELISA kit, tissue, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. tissues
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Human, Humans
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Alternative name
H. sapiens Tissue factor/coagulation factor III (thromboplastin, tissue factor) PicoKine Enzyme-linked immunosorbent assay reagent
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Alternative technique
tissues, elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
tissue
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