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ELISA s application
For quantitative measurement of human TGFBR3 in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
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Gene tested
human TGFBR3
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ELISA standard curve
Concentration(pg/ml) + 0 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.051 + 0.102 + 0.181 + 0.292 + 0.519 + 0.871 + 1.393 + 1.982
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ELISA kit detection limits
156pg/ml to 10000pg/ml
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ELISA s sensitivity
< 10pg/ml
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Target of this ELISA
Natural and recombinant human TGFBR3
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Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
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ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);1.15;2.52;5.81;1.63;2.87;5.66 ELISA standard deviations 0.051;0.108;0.284;0.109;0.169;0.401 CV(%);4.5;4.3;4.9;6.7;5.9;7.1
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ELISA kit description
GENTAUR's human TGFBR3 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for TGFBR3 was used as capure antibodies on the ELISA test plate. The internal standards (NSO,G21-D781) and your samples are measured with a biotin conjugated polyclonal from goat specific for TGFBR3 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human TGFBR3 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
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Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
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The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human TGFBR3 antibodies;1 ST0000-10;Lyophilized recombinant human TGFBR3 standard;10ng/tube×2 AR1107;Biotinylated anti- human TGFBR3 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
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Anti coagulation
heparin or EDTA
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How the elisa works
Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human TGFBR3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(heparin , EDTA) to the ELISA plate. Keep in mind that each human TGFBR3 standard solution and each sample be measured in duplicate.
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Minutes of TMB incubation
15-20
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More gene info
Betaglycan also known as Transforming growth factor beta receptor III (TGFBR3), is a cell- surfacechondroitin sulfate / heparan sulfate proteoglycan >300 kDa in molecular weight. Betaglycan binds to various members of the TGF-beta superfamily of ligands via its core protein, and bFGF via its heparan sulfate chains. It is not involved directly in TGF-beta signal transduction but by binding to various member of the TGF-beta superfamily at the cell surface it acts as a reservoir of ligand for TGF-beta receptors. By cDNA array and immunohistochemistry analyses, it is found that TGFBR3 expression is lost in most breast cancers examined in association with loss of heterozygosity of the TGFBR3 locus. TGFBR3 expression decreased during breast cancer progression, and low levels predicted decreased recurrence-free survival in patients. Loss of TGFBR3 through allelic imbalance is a frequent genetic event during breast cancer development that increases metastatic potential. Also, TGFBR3 can localize transformation in the heart and play an essential, nonredundant role in TGF-beta signaling.
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Antigen
NSO, G21-D781
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Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Uniprot
Transforming growth factor beta receptor type 3
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Gene name
transforming growth factor, beta receptor III
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Alternative
Beta glycan;Betaglycan;Betaglycan proteoglycan;BGCAN;TGBR3_HUMAN;TGF beta receptor type 3;TGF beta receptor type III;TGF-beta receptor type 3;TGF-beta receptor type III;TGFB R3;TGFBR 3;TGFBR3;TGFR 3;TGFR-3;TGFR3;Transforming growth factor beta receptor III; Transforming growth factor beta receptor III betaglycan 300kDa;Transforming growth factor beta receptor type 3
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Gen protein identity
Q03167
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Entrez nr
7049
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbol
TGFBR3
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Short name
TGFBR3 PicoKine ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Human, Humans
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Alternative name
H. sapiens transforming growth factor, b receptor III PicoKine Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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