-
ELISA s application
For quantitative measurement of human Fetuin A in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
-
Gene tested
Human Fetuin A
-
ELISA standard curve
Concentration(ng/ml) + 0 + 0.78 + 1.56 + 3.12 + 6.25 + 12.5 + 25 + 50 O.D. + 0.062 + 0.189 + 0.273 + 0.430 + 0.731 + 1.274 + 1.944 + 2.408
-
ELISA kit detection limits
0.78ng/ml to 50ng/ml
-
ELISA s sensitivity
< 10pg/ml
-
Target of this ELISA
Natural and recombinant human Fetuin A
-
Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
-
ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);0.99;5.03;18.7;1.12;5.23;23.01 ELISA standard deviations 0.046;0.221;0.953;0.084;0.361;1.703 CV(%);4.6;4.4;5.1;7.5;6.9;7.4
-
ELISA kit description
GENTAUR's human Fetuin A ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for Fetuin A was used as capure antibodies on the ELISA test plate. The internal standards (NSO, A19-V367) and your samples are measured with a biotin conjugated polyclonal from goat specific for Fetuin A will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human Fetuin A in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
-
Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
-
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human Fetuin A antibodies;1 ST0000-50;Lyophilized recombinant human Fetuin A standard;50ng/tube×2 AR1107;Biotinylated anti- human Fetuin A antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
-
Anti coagulation
heparin or EDTA
-
How the elisa works
Sample the amount of 100ul/well of the standard solution at 50ng/ml, 25ng/ml, 12.5ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml, 0.78ng/ml human Fetuin A standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma( heparin, EDTA) to the ELISA plate. Keep in mind that each human Fetuin A standard solution and each sample be measured in duplicate.
-
Minutes of TMB incubation
20-25
-
More gene info
Alpha2-HS glycoprotein(AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HS-glycoprotein(AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2
-
Antigen
NSO, A19-V367
-
Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
-
Uniprot
Alpha-2-HS-glycoprotein
-
Gene name
alpha-2-HS-glycoprotein
-
Alternative
A2HS;_x000D_AHS;_x000D_Ahsg;_x000D__x000D__x000D_Alpha 2 HS glycoprotein;_x000D_Alpha-2-HS-glycoprotein chain B;_x000D_Alpha-2-Z-globulin;_x000D_Ba-alpha-2-glycoprotein;_x000D_FETUA;_x000D_FETUA_HUMAN;_x000D_Fetuin-A;_x000D_HSGA
-
Gen protein identity
P02765
-
Entrez nr
197
-
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
-
Gene target
-
Gene symbol
AHSG
-
Short name
Fetuin A PicoKine ELISA Kit
-
Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
-
Host
antigen assay ELISA kit 1 Kit (96 Wells)
-
Species
Human, Humans
-
Alternative name
H. sapiens Fetuin A PicoKine Enzyme-linked immunosorbent assay reagent
-
Alternative technique
elisas, kits
-
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
-