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Target antigen
Hsp47
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human
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Analyses
WB,IHC-P
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Immunogen
E.coli-derived human Hsp47 recombinant protein (Position: D247-L418). Human Hsp47 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp47.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Hsp47 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Hsp47 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
Heat shock protein 47, also known as SERPINH1 or HSP47, is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. It has been found that HSP47 monitors the integrity of the triple helix of type I procollagen at the ER/cis-Golgi boundary and, when absent, the rate of transit from the ER to the Golgi is increased and the helical structure is compromised.
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Related articles
1. Christiansen, H. E., Schwarze, U., Pyott, S. M., AlSwaid, A., Al Balwi, M., Alrasheed, S., Pepin, M. G., Weis, M. A., Eyre, D. R., Byers, P. H. Homozygosity for a missense mutation in SERPINH1, which encodes the collagen chaperone protein HSP47, results in severe recessive osteogenesis imperfecta. Am. J. Hum. Genet. 86: 389-398, 2010. 2. "Entrez Gene: SERPINH1 serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)" 3. Dafforn T, Della M, Miller A (2001). "The molecular interactions of heat shock protein 47 (Hsp47) and their implications for collagen biosynthesis". J. Biol. Chem. 276(52): 49310–9.
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Gene Name
SERPINH1
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Protein Name
Serpin H1
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Gene Full Name
serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
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Synonyms
47 kDa heat shock protein antibody|47 kDa heat shock protein precursor antibody|Arsenic transactivated protein 3 antibody|Arsenic-transactivated protein 3 antibody|AsTP 3 antibody|AsTP3 antibody|CBP 1 antibody|CBP 2 antibody|CBP1 antibody|CBP2 antibody|Cell proliferation-inducing gene 14 protein antibody|Collagen binding protein 1 antibody|Collagen binding protein 2 antibody|Collagen binding protein antibody|Collagen-binding protein 2 antibody|Collagen-binding protein antibody|Colligen antibody|Colligin 1 antibody|Colligin 2 antibody|Colligin antibody|colligin-1 antibody|colligin-2 antibody|gp46 antibody|Heat shock protein 47 antibody|Heat-shock protein 47 antibody|HGNC 1547 antibody|Hsp 47 antibody|J6 antibody|OI10 antibody|PIG 14 antibody|PIG14 antibody|PPROM antibody|Proliferation inducing gene 14 antibody|Proliferation inducing gene 14 protein antibody|RA A47 antibody|RA-A47 antibody|Rheumatoid arthritis antigen A 47 antibody|rheumatoid arthritis antigen A-47 antibody|Rheumatoid arthritis related antigen RA A47 antibody|Rheumatoid arthritis-related antigen RA-A47 antibody|serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody|serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2) antibody|Serine or cysteine proteinase inhibitor clade H member 1 antibody|Serine or cysteine proteinase inhibitor clade H member 2 antibody|SERPH_HUMAN antibody|Serpin H1 antibody|Serpin peptidase inhibitor clade H member 1 antibody|serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody|Serpin peptidase inhibitor, clade H, member 1 antibody|SERPINH1 antibody|SERPINH2 antibody
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Uniprot ID
P50454
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Entrez GeneID
871
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
