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Target antigen
Eif3b
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human eIF3B(755-775aa EDFRKYRKMAQELYMEQKNER), different from the related rat and mouse sequences by one amino acid.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Eif3b Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Eif3b Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
EIF3B(Eukaryotic Translation Initiation Factor 3, Subunit B), also called PRT1 or EIF3-p116, is a protein that in humans is encoded by the EIF3B gene. By searching an EST database, Methot et al.(1997) identified a cDNA encoding a human Prt1 homolog. Asano et al.(1997) demonstrated that the 115-kD component of HeLa cell eIF3 is actually composed of 2 proteins, p116(PRT1) and an unrelated 110-kD protein. Chaudhuri et al.(1997) isolated cDNAs encoding PRT1, which they called p110 based on the size of the corresponding component of rabbit eIF3.
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Related articles
1. Asano, K., Kinzy, T. G., Merrick, W. C., Hershey, J. W. B. Conservation and diversity of eukaryotic translation initiation factor eIF3. J. Biol. Chem. 272: 1101-1109, 1997. 2. Chaudhuri, J., Chakrabarti, A., Maitra, U. Biochemical characterization of mammalian translation initiation factor 3 (eIF3): molecular cloning reveals that p110 subunit is the mammalian homologue of saccharomyces cerevisiae protein Prt1. J. Biol. Chem. 272: 30975-30983, 1997. 3. Methot, N., Rom, E., Olsen, H., Sonenberg, N. The human homologue of the yeast Prt1 protein is an integral part of the eukaryotic initiation factor 3 complex and interacts with p170. J. Biol. Chem. 272: 1110-1116, 1997.
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Gene Name
EIF3B
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Protein Name
Eukaryotic translation initiation factor 3 subunit B
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Gene Full Name
eukaryotic translation initiation factor 3, subunit B
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Synonyms
eIF-3-eta antibody|eIF3 eta antibody|eIF3 p110 antibody|eIF3 p116 antibody|EIF3-ETA antibody|EIF3-P110 antibody|EIF3-P116 antibody|eIF3b antibody|EIF3B_HUMAN antibody|EIF3S9 antibody|Eukaryotic translation initiation factor 3, subunit B antibody|Eukaryotic translation initiation factor 3 subunit 9 antibody|eukaryotic translation initiation factor 3 subunit 9 eta antibody|Eukaryotic translation initiation factor 3 subunit B antibody|hPrt1 antibody|PRT1 antibody|Prt1 homolog antibody
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Uniprot ID
P55884
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
