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Target antigen
Cytokeratin Peptide 7
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Clonality
Monoclonal antibody
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Clone
LDS-68
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Raised in
mice
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Type of the antibody
IgG1 monoclonal antibody
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Product form
freeze-dried
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Reacts with species
Human
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Analyses
WB,IHC-P,IHC-F
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Immunogen
Cytoskeletal preparation of the RT4 human bladder carcinoma cell line.
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Product configuration
Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
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Purification
Ascites
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Solubilization
The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.
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Storage condtions
Keep the Cytokeratin Peptide 7 Antibody ( LDS68)at minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Cytokeratin Peptide 7 Antibody ( LDS68) did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans.
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Related articles
1. Rosenberg, M.; Fuchs, E.; Le Beau, M. M.; Eddy, R. L.; Shows, T. B. : Three epidermal and one simple epithelial type II keratin genes map to human chromosome 12. Cytogenet. Cell Genet. 57: 33-38, 1991. 2. Smith, F. J. D.; Porter, R. M.; Corden, L. D.; Lunny, D. P.; Lane, E. B.; McLean, W. H. I. : Cloning of human, murine, and marsupial keratin 7 and a survey of K7 expression in the mouse. Biochem. Biophys. Res. Commun. 297: 818-827, 2002.
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Gene Name
KRT7
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Protein Name
Keratin, type II cytoskeletal 7
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Gene Full Name
keratin 7
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Synonyms
SCL|cytokeratin 7|K2C7|K7|keratin|Keratin-7|55K type II cytoskeletal|keratin, type II cytoskeletal 7|sarcolectin|KERATIN, SIMPLE EPITHELIAL|KERATIN, TYPE II, CYTOSKELETAL, 7|SARCOLECTIN|Type-II keratin Kb7
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Uniprot ID
Human(P08729), Mouse(Q9DCV7), Rat(Q6IG12)
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Entrez GeneID
Human(3855), Mouse(110310), Rat(300242)
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Description
Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
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French translation
anticorps
