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Target antigen
Chk1
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, rat Predicted to work with: mouse
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CChk1(69-87aa KFYGHRREGNIQYLFLEYC), identical to the related mouse and rat sequences.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Chk1 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Chk1 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
CHEK1, Cell cycle checkpoint kinase, is an enzyme that in humans is encoded by the CHEK1 gene. By fluorescence in situ hybridization,the human CHEK1 gene is mapped to 11q24, near the ATM gene at 11q23. CHEK1is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. CHEK1 acts to integrate signals from ATM and ATR, and is involved in monitoring meiotic recombination, a process that involves programmed DNA breaks.
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Related articles
1. Flaggs, G., Plug, A. W., Dunks, K. M., Mundt, K. E., Ford, J. C., Quiggle, M. R. E., Taylor, E. M., Westphal, C. H., Ashley, T., Hoekstra, M. F., Carr, A. M. Atm-dependent interactions of a mammalian Chk1 homolog with meiotic chromosomes. Curr. Biol. 7: 977-986, 1997. 2. Sanchez, Y., Wong, C., Thoma, R. S., Richman, R., Wu, Z., Piwnica-Worms, H., Elledge, S. J. Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science 277: 1497-1501, 1997. 3. Zhang, Y.-W., Otterness, D. M., Chiang, G. G., Xie, W., Liu, Y.-C., Mercurio, F., Abraham, R. T. Genotoxic stress targets human Chk1 for degradation by the ubiquitin-proteasome pathway. Molec. Cell 19: 607-618, 2005.
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Gene Name
CHEK1
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Protein Name
Serine/threonine-protein kinase Chk1
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Gene Full Name
checkpoint kinase 1
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Synonyms
C85740 antibody|Cell cycle checkpoint kinase antibody|Checkpoint , S. pombe, homolog of, 1 antibody|Checkpoint kinase 1 antibody|Checkpoint kinase 1 homolog(S. pombe) antibody|CHEK 1 antibody|Chek1 antibody|Chk 1 antibody|Chk1 antibody|CHK1 checkpoint homolog(S. pombe) antibody|CHK1_HUMAN antibody|EC 2.7.11.1 antibody|rad27 antibody|Serine/threonine protein kinase Chk1 antibody|Serine/threonine-protein kinase CHK1 antibody|STT3, subunit of the oligosaccharyltransferase complex, homolog A(S. cerevisiae) antibody
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Uniprot ID
O14757
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
