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Target antigen
Band 3
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
mouse, rat Theoretical reactivity:human
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Analyses
WB,IHC-P,IHC-F
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Immunogen
E. coli-derived human Band 3 (Position: E28-N365). Human Band 3 shares 75.7% and 74.5% amino acid (aa) sequence identity with mouse and rat Band 3, respectively.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Band 3 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Band 3 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
Band 3 is also known as SLC4A1. The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system.
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Related articles
1. Jarolim P, Palek J, Amato D, Hassan K, Sapak P, Nurse GT et al. (1991). "Deletion in erythrocyte band 3 gene in malaria-resistant Southeast Asian ovalocytosis".Proc. Natl. Acad. Sci. U.S.A. 88 (24): 11022–6. 2. Schlüter K, Drenckhahn D (August 1986)."Co-clustering of denatured hemoglobin with band 3: its role in binding of autoantibodies against band 3 to abnormal and aged erythrocytes". Proc. Natl. Acad. Sci. U.S.A. 83 (16): 6137–41.
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Gene Name
SLC4A1
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Protein Name
Band 3 anion transport protein
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Gene Full Name
solute carrier family 4 (anion exchanger), member 1 (Diego blood group)
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Synonyms
AE 1 antibody|AE1 antibody|Anion exchange protein 1 antibody|Anion exchanger 1 antibody| B3AT_HUMAN antibody|Band 3 anion transport protein antibody|BND3 antibody|CD233 antibody|DI antibody|Diego blood group antibody|EMPB3 antibody|EPB3 antibody|Erythrocyte membrane protein band 3 antibody|Erythroid anion exchange protein antibody|FR antibody|Froese blood group antibody|RTA1A antibody|SLC4A1 antibody|Solute carrier family 4 anion exchanger member 1 antibody|Solute carrier family 4 member 1 antibody|SW antibody|Swann blood group antibody|Waldner blood group antibody|WD antibody|WD1 antibody|WR antibody|Wright blood group antibody
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Uniprot ID
P02730
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Entrez GeneID
6521
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
