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Target antigen
B Raf
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
mouse, rat
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Analyses
WB
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Immunogen
E.coli-derived human B Raf recombinant protein (Position: A38-V230). Human B Raf shares 81% amino acid (aa) sequence identity with mouse B Raf.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the B Raf Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The B Raf Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf. It is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. It is mapped to 7q34. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. The BRAF protein is also involved in sending signals inside cells, which are involved in directing cell growth.
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Related articles
1. Sithanandam G, Kolch W, Duh FM, Rapp UR (December 1990). "Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies". Oncogene 5 (12): 1775–80. 2. Sithanandam G, Druck T, Cannizzaro LA, Leuzzi G, Huebner K, Rapp UR (April 1992). "B-raf and a B-raf pseudogene are located on 7q in man". Oncogene 7 (4): 795–9. 3. Sithanandam, G., Kolch. W., Duh, F.-M., Rapp, U. R.Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies.Oncogene 5: 1775-1780, 1990.
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Gene Name
BRAF
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Protein Name
Serine/threonine-protein kinase B-raf
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Gene Full Name
B-Raf proto-oncogene, serine/threonine kinase
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Synonyms
FLJ95109 antibody|94 kDa B raf protein antibody|B raf 1 antibody|B raf 1 antibody|B Raf proto oncogene serine threonine protein kinase antibody|B Raf proto oncogene serine threonine protein kinase antibody|B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody|BRAF 1 antibody|BRaf antibody|BRaf antibody|BRAF_HUMAN antibody|BRAF1 antibody|BRAF1 antibody|cRmil antibody|MGC126806 antibody|MGC138284 antibody|Murine sarcoma viral (v-raf) oncogene homolog B1 antibody|Murine sarcoma viral (v-raf) oncogene homolog B1 antibody|Murine sarcoma viral v raf oncogene homolog B1 antibody|Murine sarcoma viral v raf oncogene homolog B1 antibody|NS7 antibody|oncogene BRAF1 antibody|p94 antibody|p94 antibody|Proto-oncogene B-Raf antibody|Proto-oncogene c-Rmil antibody|RAFB 1 antibody|RAFB1 antibody|RAFB1 antibody|RMIL antibody|Serine/threonine-protein kinase B-raf antibody|v raf murine sarcoma viral oncogene homolog B antibody|v raf murine sarcoma viral oncogene homolog B1 antibody|v raf murine sarcoma viral oncogene homolog B1 antibody|v-Raf murine sarcoma viral oncogene homolog B1 antibody
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Uniprot ID
P15056
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Entrez GeneID
673
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
