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Target antigen
B Raf
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human Predicted to work with: mouse
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human B Raf(344-362aa QPFRPADEDHRNQFGQRDR), identical to the related rat and mouse sequences.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the B Raf Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The B Raf Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
BRAF(v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf, also known as ONCOGENE BRAF, BRAF1, RAFB1, Proto-oncogene B-Raf p94, v-Raf murine sarcoma viral oncogene homolog B1, proto-oncogene B-Raf, v-Raf murine sarcoma viral oncogene homolog B1, while the protein is more formally known as serine/threonine-protein kinase B-Raf. BRAF deduced 651-amino acid protein has a calculated molecular mass of 72.5 kD. Northern blot analysis detected transcripts of 10 and 13 kb in cerebrum, fetal brain, and placenta and transcripts of 2.6 and 4.5 kb in testis. Testis also showed lower expression of the 10- and 13-kb transcripts.B-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion.
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Related articles
1. Eychene, A., Barnier, J. V., Apiou, F., Dutrillaux, B., Calothy, G. Chromosomal assignment of two human B-raf(Rmil) proto-oncogene loci: B-raf-1 encoding the p94(Braf/Rmil) and B-raf-2, a processed pseudogene.Oncogene 7: 1657-1660, 1992. 2. Hishinuma, A., Fukata, S., Kakudo, K., Murata, Y., Ieiri, T. High incidence of thyroid cancer in long-standing goiters with thyroglobulin mutations. Thyroid 15: 1079-1084, 2005. 3. Sithanandam, G., Kolch. W., Duh, F.-M., Rapp, U. R.Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies.Oncogene 5: 1775-1780, 1990.
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Gene Name
BRAF
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Protein Name
Serine/threonine-protein kinase B-raf
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Gene Full Name
v-raf murine sarcoma viral oncogene homolog B1
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Synonyms
FLJ95109 antibody|94 kDa B raf protein antibody|B raf 1 antibody|B Raf proto oncogene serine threonine protein kinase antibody|BRAF 1 antibody|BRAF antibody|BRAF_HUMAN antibody|BRAF1 antibody|cRmil antibody|MGC126806 antibody|MGC138284 antibody|Murine sarcoma viral(v-raf) oncogene homolog B1 antibody|Murine sarcoma viral v raf oncogene homolog B1 antibody|NS7 antibody|p94 antibody|Proto-oncogene B-Raf antibody|Proto-oncogene c-Rmil antibody|RAFB 1 antibody|RAFB1 antibody|Serine/threonine-protein kinase B-raf antibody|v raf murine sarcoma viral oncogene homolog B1 antibody|v-Raf murine sarcoma viral oncogene homolog B1 antibody
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Uniprot ID
P15056
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
