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Target antigen
Aromatase
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, rat Predicted to work with: mouse
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Analyses
WB,IHC-P
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Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Aromatase(485-503aa KNMLEMIFTPRNSDRCLEH).
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Aromatase Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Aromatase Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
Aromatase is an enzyme responsible for a key step in the biosynthesis of estrogens. It is a member of the cytochrome P450 superfamily, which are monooxygenases that catalyze many reactions involved in steroidogenesis. In particular, aromatase is responsible for the aromatization of androgens into estrogens. The CYP19 gene spans at least 70 kb of genomic DNA and contains 10 exons. By in situ hybridization, the ARO gene is mapped to 15q21.1. The aromatase enzyme can be found in many tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, and endometrium, as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer. It is an important factor in sexual development. Some bodybuilders taking steroids also take antiaromatase supplements to prevent excess testosterone conversion into estrogens, which can cause gynecomastia.
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Related articles
1. Chen, S., Besman, M. J., Sparkes, R. S., Zollman, S., Klisak, I., Mohandas, T., Hall, P. F., Shively, J. E.Human aromatase: cDNA cloning, Southern blot analysis, and assignment of the gene to chromosome 15.DNA 7: 27-38, 1988. 2. Harada, N., Ogawa, H., Shozu, M., Yamada, K., Suhara, K., Nishida, E., Takagi, Y.Biochemical and molecular genetic analyses on placental aromatase (P-450-AROM) deficiency.J. Biol. Chem. 267: 4781-4785, 1992. 3. Zhou, D., Pompon, D., Chen, S.Structure-function studies of human aromatase by site-directed mutagenesis: kinetic properties of mutants pro308-to-phe, tyr361-to-phe, tyr361-to-leu, and phe406-to-arg.Proc. Nat. Acad. Sci. 88: 410-414, 1991.
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Gene Name
CYP19A1
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Protein Name
Aromatase
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Gene Full Name
cytochrome P450, family 19, subfamily A, polypeptide 1
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Synonyms
ARO 1 antibody|ARO antibody|ARO1 antibody|Aromatase antibody|CP19A_HUMAN antibody|CPV antibody|CPV1 antibody|CYAR antibody|CYP19 antibody|CYP19A1 antibody|CYPXIX antibody|Cytochrome P-450AROM antibody|Cytochrome P450 19A1 antibody|Cytochrome P450 family 19 subfamily A polypeptide 1 antibody|Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody|Cytochrome P450, subfamily XIX(aromatization of androgens) antibody|Estrogen synthase antibody|Estrogen synthetase antibody|Flavoprotein linked monooxygenase antibody|MGC104309 antibody|Microsomal monooxygenase antibody|OTTHUMP00000162543 antibody|OTTHUMP00000198350 antibody|P 450AROM antibody|P450AROM antibody
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Uniprot ID
P11511
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
