Alkaline Phosphatase Conjugated anti-Mouse/Rabbit IgG SABC Kit
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Catalog numberSA1050
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Price:
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Size1 kit
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ImmunogenNA
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Storage Transport Conditions4°C for one year. Avoid freezing
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Kit Components1. 5% BSA Blocking Reagent: 12 ml, for blocking tissue sections. 2. Biotinylated Secondary Antibody (Goat Anti-mouse/anti-rabbit IgG): 12ml (10ug/ml). Affinity purified antibody, labeled with “long-arm†biotin (Biotinamidohexanoic acid N-hydroxysuccinimide ester, CAS# 72040-63-2). 3. SABC-AP (Alkaline Phosphatase conjugated streptavidin): 12ml (20ug/ml). Manufactured by Boster’s proprietary method, the complex is very stable and offers superior amplification of the antigen signals. 4. BCIP/NBT Chromogenic agent (20x): 1ml. 5. Water soluble sealing reagent: 12 ml. 6. Neutral nuclear fast red: 12 ml.
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ApplicationsIHC, ICC
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ReactivityNA
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by boster. Rabbit antibodies are very stable and can be stored for several days at room temperature. boster adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Oryctolagus cuniculus
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Gene target
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Short nameAlkaline Phosphatase Conjugated anti-Mouse/Rabbit IgG SABC Kit
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TechniqueRabbit, anti-, Mouse, anti, IgG, antibody to, antibodies, antibody Conjugates, IgGs, mouses
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HostGoat, Rabbits
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IsotypeIgG, IgG
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LabelAlkaline Phosphatase
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SpeciesMouse, Mouses
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Alternative nameAlkaline Phosphatase coupled Antibody toMouse/production species: rabbit Immunoglobulin G SABC reagent
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Alternative techniquerabbit-anti, murine, antibodies, kits, immunoglobulins
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data