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Target antigen
ABCG5
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, rat
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ABCG5 (197-221aa ERRRVSIAAQLLQDPKVMLFDEPTT), different from the related mouse and rat sequences by two amino acids.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the ABCG5 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The ABCG5 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
ABCG5 (Atp-binding cassette, subfamily g, member 5) also known as STEROLIN 1, is a protein that in humans is encoded by the ABCG5 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. The ABCG5 gene contains 13 exons and spans about 28 kb. The ABCG5 gene is mapped on 2p21. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. Small (2003) reviewed the role of ABC transporters in secretion of cholesterol from liver into bile, particularly the role of ABCG5/ABCG8. The ABCG5 and ABCG8 genes are an example of closely neighboring genes in a head-to-head orientation that, when mutated, cause the same phenotype.
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Related articles
1. Lee, M.-H., Lu, K., Hazard, S., Yu, H., Shulenin, S., Hidaka, H., Kojima, H., Allikmets, R., Sakuma, N., Pegoraro, R., Srivastava, A. K., Salen, G., Dean, M., Patel, S. B. Identification of a gene, ABCG5, important in the regulation of dietary cholesterol absorption. Nature Genet. 27: 79-83, 2001. 2. Lu, K., Lee, M. H., Yu, H., Zhou, Y., Sandell, S. A., Salen, G., Patel, S. B. Molecular cloning, genomic organization, genetic variations, and characterization of murine sterolin genes Abcg5and Abcg8. J. Lipid Res. 43: 565-578, 2002. 3. Small, D. M. Role of ABC transporters in secretion of cholesterol from liver into bile. (Commentary)Proc. Nat. Acad. Sci. 100: 4-6, 2003.
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Gene Name
ABCG5
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Protein Name
ATP-binding cassette sub-family G member 5
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Gene Full Name
ATP-binding cassette, sub-family G (WHITE), member 5
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Synonyms
ABCG5 antibody|ABCG5_HUMAN antibody|ATP binding cassette sub family G (WHITE) member 5 (sterolin 1) antibody|ATP binding cassette sub family G member 5 antibody|ATP-binding cassette sub-family G member 5 antibody|Sterolin 1 antibody|Sterolin-1 antibody|STSL antibody
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Uniprot ID
Q9H222
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Entrez GeneID
64240
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
