143
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Catalog numberPA1797
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PricePlease ask
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Size0,1 mg
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Target antigen143
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ClonalityPolyclonal antibody
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ClonePolyclonal antibody
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Raised inrabbit
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Type of the antibodyIgG polyclonal antibody
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Product formfreeze-dried
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Reacts with specieshuman
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AnalysesWB,IHC-P,ICC
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ImmunogenA synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 sigma(140-156aa KKRIIDSARSAYQEAMD), identical to the related rat and mouse sequences.
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Product configurationEach vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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PurificationImmunogen affinity purified.
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SolubilizationThe powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtionsKeep the 143at minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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TipsThe 143 did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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BackgroundStratifin(SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al.(1993)obtained peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.
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Related articles1. Leffers, H., Madsen, P., Rasmussen, H. H., Honore, B., Andersen, A. H., Walbum, E., Vandekerckhove, J., Celis, J. E.Molecular cloning and expression of the transformation sensitive epithelial marker stratifin: a member of a protein family that has been involved in the protein kinase C signalling pathway.J. Molec. Biol. 231: 982-998, 1993. 2. Urano, T., Saito, T., Tsukui, T., Fujita, M., Hosoi, T., Muramatsu, M., Ouchi, Y., Inoue, S. Efp targets 14-3-3-sigma for proteolysis and promotes breast tumour growth.Nature 417: 871-875, 2002. 3. Wilker, E. W., van Vugt, M. A. T. M., Artim, S. A., Huang, P. H., Petersen, C. P., Reinhardt, H. C., Feng, Y., Sharp, P. A., Sonenberg, N., White, F. M., Yaffe, M. B.14-3-3-sigma controls mitotic translation to facilitate cytokinesis.Nature 446: 329-332, 2007.
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Gene NameSFN
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Protein Name14-3-3 protein sigma
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Gene Full Namestratifin
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Synonyms14 3 3 protein sigma antibody|14-3-3 protein sigma antibody|1433S_HUMAN antibody|Epithelial cell marker protein 1 antibody|Er antibody|HME 1 antibody|HME1 antibody|MGC143283 antibody|Mkrn3 antibody|Mme1 antibody|OTTHUMP00000004242 antibody|RP23 137L22.11 antibody|SFN antibody|SFN protein antibody|Stratifin antibody|YWHAS antibody
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Uniprot IDP31947
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Gene target
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Gene symbolMIR143, RN7SKP143, RNA5SP143
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Short name143
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Alternative name143
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Gene info
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Identity
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Gene
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Long gene namemicroRNA 143
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNAs
Gene info
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Identity
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Gene
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Long gene nameRN7SK pseudogene 143
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Synonyms gene name
- RNA, 7SK small nuclear pseudogene 143
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Locus
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Discovery year2013-03-19
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene nameRNA, 5S ribosomal pseudogene 143
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Synonyms gene
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Synonyms gene name
- RNA, 5S ribosomal 143
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Locus
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Discovery year2011-10-07
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Entrez gene record
