Recombinant Thermobifida Fusca Cutinase(C-6xHis)

Alternative names

Cutinase
Description

Recombinant Thermobifida Fusca Cutinase is produced by our E.coli expression system and the target gene encoding Ala1-Phe261 is expressed with a 6His tag at the C-terminus. .
Expression

E. coli
Tag

C-His
Endotoxin

Less than 0.1 ng/ug (1 EU/ug)
Accession

E5BBQ3
TargetSequence

Ala1-Phe261
Shipping

Dry ice/Polar packs
Formulation

Supplied as a 0.2 μm filtered solution of 10mM Tris-HCl,1mM 2-mercaptoethanol,2mM MnCl2,150mM NaCl,pH7.5.
Note

For research use only.
Conjugated

The polyhistidine (6xHis) tag or Histidine Epitope Tag (HIS Tag, 6XHIS Tag with Histidine (abbreviated as His or H; encoded by the codons CAU and CAC) is an α-amino acid that is used in the biosynthesis of proteins. It is the most used tag for purification by Affinity Chromatography (IMAC). The pentadentate chelator is the best chelator in Immobilized Metal ion Affinity chromatography (IMAC) for the6xhis-tagged proteins.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

Thermobifida Fusca Cutinase
Short name

Recombinant Thermobifida Fusca Cutinase(C- )
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Bon Opus advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Species

T. fusca
Alternative name

Rec. Thermobifida Fusca Cutinase(C-6xHis)
Alternative technique

rec

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data