Recombinant Mouse CD40 ligand/CD40LG (N-6His)

Alternative names

CD40 Ligand; CD40LG;HIGM1; T-B cell-activating molecule; T-BAM; TNFSF5; tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5
Description

Recombinant Mouse CD40 ligand is produced by our Mammalian expression system and the target gene encoding Met112-Leu260 is expressed with a 6His tag at the N-terminus.
Expression

Human Cells
Tag

N-His
Endotoxin

Less than 0.1 ng/ug (1 EU/ug)
Accession

P27548
TargetSequence

Met112-Leu260
Shipping

Ambient
Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Note

For research use only.
Additional description

FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name

Mus musculus
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

CD40 ligand/CD40LG N-6His
Gene symbol

CD40, CD40LG
Short name

Recombinant Mouse CD40 ligand/CD40LG (N-6His)
Technique

Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Bon Opus advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Host

mouse
Species

Mouse, Mouses
Alternative name

Rec. Mouse CD40 molecule, tumor necrosis factor receptor superfamily member 5 ligand/CD40 molecule, tumor necrosis factor receptor superfamily member 5 ligand (N-6His)
Alternative technique

rec, murine
Alternative to gene target

CD40 molecule, TNF receptor superfamily member 5, Bp50 and CDW40 and p50 and TNFRSF5, CD40 and IDBG-78904 and ENSG00000101017 and 958, ubiquitin protein ligase binding, Extracellular, Cd40 and IDBG-212533 and ENSMUSG00000017652 and 21939, BT.42498 and IDBG-643036 and ENSBTAG00000020736 and 286849

Identity

HGNC:11919
Gene

CD40
Long gene name

CD40 molecule
Synonyms gene
- TNFRSF5
Synonyms gene name
- tumor necrosis factor receptor superfamily, member 5
- CD40 molecule, TNF receptor superfamily member 5
Synonyms
- p50
- Bp50
GenBank acession
- X60592
Locus

20q13.12
Discovery year

1993-08-27
Entrez gene record

958
Pubmed identfication
- 7687385
- 2998589
RefSeq identity
- NM_001250
Classification
- CD molecules
- Tumor necrosis factor receptor superfamily
VEGA ID

OTTHUMG00000033053
Locus Specific Databases
- CD40base: Mutation registry for CD40 deficiency (previously known as TNFRSF5base)
- LRG_40

Identity

HGNC:11935
Gene

CD40LG
Long gene name

CD40 ligand
Synonyms gene
- HIGM1
- IMD3
- TNFSF5
Synonyms gene name
- tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
Synonyms
- CD40L
- TRAP
- gp39
- hCD40L
- CD154
Synonyms name
GenBank acession
- X67878
Locus

Xq26.3
Discovery year

1989-06-30
Entrez gene record

959
Pubmed identfication
- 1427881
- 7678782
RefSeq identity
- NM_000074
Classification
- CD molecules
- Tumor necrosis factor superfamily
VEGA ID

OTTHUMG00000022512
Locus Specific Databases

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data