Recombinant Human Brain Natriuretic Peptide/BNP (N-6His-Flag)

• Catalog number
  CM29
• Price
  Please ask
• Size
  50ug

• Alternative names
  Natriuretic peptides B, Gamma-brain natriuretic peptide, NPPB, BNP
• Description
  Recombinant Human Brain-type Natriuretic Peptide is produced by our E.coli expression system and the target gene encoding His27-Arg102 is expressed with a 6His, Flag tag at the N-terminus.
• Expression
  E. coli
• Tag
  N-His&Flag
• Endotoxin
  Less than 0.1 ng/ug (1 EU/ug)
• Accession
  P16860
• TargetSequence
  His27-Arg102
• Shipping
  Ambient
• Formulation
  Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.
• Note
  For research use only.
• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Flag
• Additional description
  Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Brain   Natriuretic   Peptide/BNP   N-6His  
• Short name
  Recombinant Brain Natriuretic Peptide/BNP (N-6His- )
• Technique
  Recombinant, peptide, peptides, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Bon Opus advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Label
  Flag
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Brain Natriuretic short protein sequence/BNP (N-6His-Flag)
• Alternative technique
  rec, peptides
• Tissue
  brain

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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