Recombinant Human Alpha-enolase/Enolase 1/MPB-1/NNE(C-6His)

Alternative names

Alpha-enolase;2-phospho-D-glycerate hydro-lyase;C-myc promoter-binding protein;Enolase 1;MBP-1;MPB-1;Non-neural enolase;NNE;Phosphopyruvate hydratase;Plasminogen-binding protein;ENO1
Description

Recombinant Human Alpha-enolase is produced by our E.coli expression system and the target gene encoding Met1-Lys434 is expressed with a 6His tag at the C-terminus.
Expression

E. coli
Tag

C-His
Endotoxin

Less than 0.1 ng/ug (1 EU/ug)
Accession

P06733
TargetSequence

Met1-Lys434
Shipping

Dry ice/Polar packs
Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 250mM NaCl, 1mM MgSO4,20%glycerol，Ph7.5 .
Note

For research use only.
Additional description

The Recombinant Alpha-enolase/Enolase 1/MPB-1/NNE(C-6His) is a α- or alpha protein sometimes glycoprotein present in blood.
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

Alpha-enolase/Enolase 1/MPB-1/NNE C-6His
Gene symbol

ENO1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1, MIR16-1
Short name

Recombinant Alpha-enolase/Enolase 1/MPB-1/NNE(C-6His)
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Bon Opus advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Species

Human, Humans
Alternative name

Rec. H. sapiens a-enolase/Enolase 1/MPB-1/NNE(C-6His)
Alternative technique

rec

Identity

HGNC:5670
Gene

IGHVII-1-1
Long gene name

immunoglobulin heavy variable (II)-1-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
Synonyms
- IGHV(II)-1-1
GenBank acession
- AB019441
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28377
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000152325

Identity

HGNC:5760
Gene

IGKV1OR2-1
Long gene name

immunoglobulin kappa variable 1/OR2-1 (pseudogene)
Synonyms gene
- IGKVPZ1
- IGKV1OR-1
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
Synonyms
- IGKV1/OR-1
- IGKV1/OR2-1
GenBank acession
- Z12367
Locus

2p11.1
Discovery year

2000-04-18
Entrez gene record

3531
Pubmed identfication
- 2853098
Classification
- Immunoglobulin kappa (IGK) orphons
VEGA ID

OTTHUMG00000155081

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data