Recombinant E.coli RNA-splicing ligase RtcB
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Catalog number
BP104
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Price
Please ask
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Size
500ug
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Alternative names
3'-phosphate/5'-hydroxy nucleic acid ligase; rtcB; yhgL
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Description
Recombinant E.coli RNA-splicing ligase RtcB is produced by E.coli. The target gene encoding M1-G408 is expressed.
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Expression
E. coli
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Tag
tag free
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Endotoxin
Less than 0.1 ng/ug (1 EU/ug)
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Accession
P46850
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TargetSequence
M1-G408
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Shipping
Dry ice/ice pack
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Formulation
Supplied as a 0.2 um filtered solution of 2umM HEPES, 30umM NaCl,10%glycerol, umM DTT,0.umM EDTA,pH7.5.
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Note
For research use only.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
Recombinant E coli RNA-splicing ligase RtcB
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Bon Opus advises they will be reconstituted in a buffer soluion or culture medium for cell culture. RNAs are to store at -70 C
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Host
Escherichia coli
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Species
E. coli
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Alternative name
Rec. Escherichia Coli RNA-splicing ligase RtcB
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Alternative technique
rec, escherichia
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MeSH Data
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Name
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Concept
Scope note:
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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