ZAP70 (4B2) monoclonal antibody

• Catalog number
  MB0187-100ul
• Price
  Please ask
• Size
  100ul

• Alternative Name
  Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase; ZAP70; SRK
• Immunogen
  Purified recombinant human ZAP70 protein fragments expressed in E.coli.
• Swiss Prot
  P43403
• Reactivity
  Human
• Applications
  WB IP
• Application_all
  WB: 1:1000 IP: 1:50~200
• Product
  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  This antibody detects endogenous levels of ZAP70 and does not cross-react with related proteins.
• Usage
  Western blot detection of ZAP-70 in CEM and Jurkat cell lysates,negative in the Ramos cell lysates using ZAP-70 mouse mAb (1:1000 diluted).
• BiowMW
  Predicted band size:70KDa Observed band size:70KDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  ZAP70   4B2   monoclonal  
• Gene symbol
  ZAP70
• Short name
  ZAP70 (4B2) monoclonal antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse
• Alternative name
  zeta-chain (TCR) associated protein kinase 70kDa (4B2) monoclonal (antibody to-)
• Alternative technique
  antibodies
• Alternative to gene target
  zeta-chain (TCR) associated protein kinase 70kDa, SRK and STCD and STD and TZK and ZAP-70, ZAP70 and IDBG-62509 and ENSG00000115085 and 7535, transferase activity, Cell surfaces, Zap70 and IDBG-145089 and ENSMUSG00000026117 and 22637, ZAP70 and IDBG-629842 and ENSBTAG00000005892 and 504509

Gene info

• Identity
  HGNC:12858
• Gene
  ZAP70
• Long gene name
  zeta chain of T cell receptor associated protein kinase 70
• Synonyms gene
  • SRK
• Synonyms gene name
  • SRK
• Synonyms
  • ZAP-70
  • STD
• Synonyms name
  • tyrosine-protein kinase ZAP-70
• GenBank acession
  • L05148
• Locus
  2q11.2
• Discovery year
  1995-05-16
• Entrez gene record
  7535
• Pubmed identfication
  • 1423621
• RefSeq identity
  • NM_001079
• Classification
  • SH2 domain containing
  • Syk family tyrosine kinases
• VEGA ID
  OTTHUMG00000153060
• Locus Specific Databases
  • ZAP70base: Mutation registry for autosomal recessive ZAP70 immunodeficiency
  • LRG_126

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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