TGFα polyclonal antibody

Alternative Name

TGF-alpha, ETGF, TGFA, EGF like TGF, EGF-like TGF, ETGF, TFGA, TGF A, TGF type 1, TGF-alpha, Tgfa, TGFA, Transforming growth factor alpha, Transforming growth factor alpha precursor, Wa1, Waved 1,
Immunogen

Recombinant protein
Swiss Prot

P01135(Human)
Reactivity

Human
Applications

WB ICC IF IHC IP
Application_all

WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200
Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification Purity

ProA affinity purified
Storage Stability

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity

TGFα polyclonal antibody detects endogenous levels of TGFα protein.
Usage

ICC staining TGF alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
BiowMW

17 kDa
Note

For research use only, not for use in diagnostic procedure.
Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group

Polyclonals and antibodies
About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation

anticorps

Gene target

TGFα
Gene symbol

TBILA, LTBP1, TAB3
Short name

TGFα polyclonal antibody
Technique

Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
Host

Rabbit
Alternative name

TGFα polyclonal (antibody to-)
Alternative technique

polyclonals, antibodies

Identity

HGNC:30681
Gene

TAB3
Long gene name

TGF-beta activated kinase 1 (MAP3K7) binding protein 3
Synonyms gene
- MAP3K7IP3
Synonyms gene name
- mitogen-activated protein kinase kinase kinase 7 interacting protein 3
- TGF-beta activated kinase 1/MAP3K7 binding protein 3
- TGF-beta activated kinase 1 and MAP3K7 binding protein 3
Synonyms name
- TAK1 binding protein 3
GenBank acession
- AY331591
Locus

Xp21.2
Discovery year

2006-06-26
Entrez gene record

257397
Pubmed identfication
- 14633987
- 14670075
RefSeq identity
- NM_152787
Classification
- Zinc fingers RANBP2-type
VEGA ID

OTTHUMG00000021329
Locus Specific Databases
- Global Variome shared LOVD

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data