Stat1 (phospho-S727) polyclonal antibody

• Catalog number
  BS5036-100ul
• Price
  Please ask
• Size
  100ul

• Alternative Name
  Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84; STAT1; STAT1α; STAT1β
• Immunogen
  Synthetic phosphopeptide derived from human Stat1 around the phosphorylation site of Serine 727.
• Swiss Prot
  P42224
• Reactivity
  Human,Mouse,Rat
• Applications
  WB IHC
• Application_all
  WB: 1:500~1:1000 IHC: 1:50~1:200
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  p-Stat1 (S727) polyclonal antibody detects endogenous levels of Stat1 protein only when phosphorylated at Ser727.
• Usage
  Western blot (WB) analysis of Stat1 (phospho-S727) polyclonal antibody at 1:500 dillution Lane1:NIH-3T3 whole cell lysate(40μg) Lane2:NIH-3T3 treated with UV(4h) whole cell lysate(40μg) Lane3:NIH-3T3 treated with PMA(60ng/ml, 30min) whole cell lysate(40μg) Lane4:Hela whole cell lysate(40μg) Lane5:Hela treated with IFN-γ(100ng/ml, 15min) whole cell lysate(40μg) Lane6:Hela treated with PMA(60ng/ml, 30min) whole cell lysate(40μg)
• BiowMW
  ~ 87 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  Stat1   phospho-S727  
• Short name
  Stat1 (phospho-S727) polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit
• Alternative name
  signal transducer and activator on transcription 1, 91kDa (phosphorilated-S727) polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies
• Alternative to gene target
  signal transducer and activator of transcription 1, 91kDa, CANDF7 and IMD31A and IMD31B and IMD31C and ISGF-3 and STAT91, STAT1 and IDBG-77617 and ENSG00000115415 and 6772, sequence-specific DNA binding, nuclei, Stat1 and IDBG-153162 and ENSMUSG00000026104 and 20846, STAT1 and IDBG-642654 and ENSBTAG00000007867 and 510814,515988

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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