Smad2/3 (N175) polyclonal antibody
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Catalog numberAP0445-50ul
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Price:
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Size50ul
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Alternative NameMothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2
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ImmunogenSynthetic peptide, corresponding to amino acids 145-195 of Human Smad2.
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Swiss ProtP84022/Q15796
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ReactivityHuman,Mouse,Rat
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ApplicationsWB
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Application_allWB: 1:1000~1:2000
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ProductRabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
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Storage StabilityStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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SpecificitySmad2/3 (N175) polyclonal antibody detects endogenous levels of Smad2/3 protein.
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UsageWestern blot (WB) analysis of Smad2/3 (N175) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate
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BiowMW~ 55,60 kDa
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NoteFor research use only, not for use in diagnostic procedure.
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PropertiesIf you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translationanticorps
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Gene target
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Short nameSmad2/3 (N175) polyclonal antibody
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TechniquePolyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit
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Alternative nameSMAD family member 2/3 (N175) polyclonal (antibody to-)
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Alternative techniquepolyclonals, antibodies
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Alternative to gene targetSMAD family member 2, hMAD-2 and hSMAD2 and JV18 and JV18-1 and MADH2 and MADR2, SMAD2 and IDBG-3127 and ENSG00000175387 and 4087, transforming growth factor beta receptor, nuclei, Smad2 and IDBG-159056 and ENSMUSG00000024563 and 17126, SMAD2 and IDBG-644642 and ENSBTAG00000039916 and 515125,516010
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data