PKC α (phospho-T638) polyclonal antibody
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Catalog numberAP0290-50ul
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Price:
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Size50ul
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Alternative NameProtein kinase C alpha type; PKC-A; PKC-alpha; PRKCA; PKCA; PRKACA
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ImmunogenSynthetic phosphopeptide derived from human PKC α around the phosphorylation site of Threonine 638.
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Swiss ProtP17252
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ReactivityHuman,Mouse,Rat
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ApplicationsWB IHC IP
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Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100
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ProductRabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).
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Storage StabilityStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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SpecificityPKC α (phospho-T638) polyclonal antibody detects endogenous levels of PKC α protein when phosphorylated at Thr638.
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UsageWestern blot (WB) analysis of PKC α (phospho-T638) polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:MCF-7 treated with LPS(100ng/ml) for 30 minutes whole cell lysate Lane4:MCF-7 treated with LPS(100ng/ml) for 5 minutes whole cell lysate Lane5:MCF-7 whole cell lysate
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BiowMW~ 80 kDa
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NoteFor research use only, not for use in diagnostic procedure.
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PropertiesIf you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translationanticorps
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Gene target
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Gene symbolPRKCH, PRKD1, PRRT2, PRKDC
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Short namePKC α (phospho-T638) polyclonal antibody
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TechniquePolyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit
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Alternative namePKC α (phosphorilated-T638) polyclonal (antibody to-)
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameprotein kinase C eta
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Synonyms gene
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Synonyms gene name
- protein kinase C, eta
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-06-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C2 domain containing protein kinases
- AGC family kinases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein kinase D1
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Synonyms gene
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Synonyms gene name
- protein kinase C, mu
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Synonyms
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Locus
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Discovery year1994-05-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Pleckstrin homology domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameproline rich transmembrane protein 2
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Synonyms gene
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Synonyms gene name
- infantile convulsions and paroxysmal choreoathetosis
- dystonia 10
- proline-rich transmembrane protein 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-11-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- interferon induced transmembrane protein domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein kinase, DNA-activated, catalytic subunit
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Synonyms gene
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Synonyms gene name
- protein kinase, DNA-activated, catalytic polypeptide
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Synonyms
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Synonyms name
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Locus
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Discovery year1993-11-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data