p70 S6K (phospho-T421) polyclonal antibody

• Catalog number
  BS4853-100ul
• Price
  Please ask
• Size
  100ul

• Alternative Name
  Ribosomal protein S6 kinase I; S6K; S6K1; 70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase alpha; p70 S6K; p70-S6K; P70S6K; p70 S6 kinase α; Name: RPS6KB1; STK14A; p80 S6K1; p85 S6 kinase; p85 S6K; p85S6K
• Immunogen
  Synthetic phosphopeptide derived from human p70 S6K around the phosphorylation site of Threonine 421.
• Swiss Prot
  P23443
• Reactivity
  Human,Mouse,Rat
• Applications
  WB IHC
• Application_all
  WB:1:500~1:1000 IHC:1:50~1:200
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  p-p70 S6K (T421) polyclonal antibody detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr421. This antibody also detects p85 S6 kinase when phosphorylated at Thr444.
• Usage
  Western blot (WB) analysis of p-p70 S6K (T421) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with PMA(100nM,15mins) Lane2:NIH-3T3 cell lysate treated with PMA(100nM,15mins) Lane3:H9C2 cell lysate treated with PMA(100nM,15mins)
• BiowMW
  ~ 59,70,85 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  p70   S6K   phospho-T421  
• Gene symbol
  RPS6KB1
• Short name
  p70 S6K (phospho-T421) polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit
• Alternative name
  p70 S6K (phosphorilated-T421) polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:10436
• Gene
  RPS6KB1
• Long gene name
  ribosomal protein S6 kinase B1
• Synonyms gene
  • STK14A
• Synonyms gene name
  • ribosomal protein S6 kinase, 70kD, polypeptide 1
• Synonyms
  • S6K1
  • p70(S6K)-alpha
  • PS6K
  • S6K
• GenBank acession
  • M60724
• Locus
  17q23.1
• Discovery year
  1994-07-11
• Entrez gene record
  6198
• Pubmed identfication
  • 1922062
• RefSeq identity
  • NM_003161
• Classification
  • AGC family kinases
• VEGA ID
  OTTHUMG00000150642

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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