MSK1 (phospho-Thr581) polyclonal antibody
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Catalog numberAP0095-50ul
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Price:
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Size50ul
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Alternative NameRibosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL; RPS6KA5; MSK1
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ImmunogenSynthetic phosphopeptide derived from human MSK1 around the phosphorylation site of Threonine 581.
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Swiss ProtO75582
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ReactivityHuman,Mouse,Rat
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ApplicationsWB
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Application_allWB: 1:500~1:2000
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ProductRabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).
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Storage StabilityStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Specificityp-MSK1(Thr581) polyclonal antibody detects endogenous levels of MSK1 protein when phosphorylated at Thr581.
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UsageWestern blot (WB) analysis of p-MSK1(Thr581) polyclonal antibody at 1:500 or 1:2000 dilution Lane1:H9C2 treated with LPS(40ng/ml) for 45min whole cell lysate Lane2:H9C2 treated with LPS(40ng/ml) for 15min whole cell lysate Lane3:H9C2 treated with LPS(40ng/ml) for 5min whole cell lysate Lane4:L02 treated with LPS(40ng/ml) for 45min whole cell lysate Lane5:L02 treated with LPS(40ng/ml) for 15min whole cell lysate Lane6:L02 whole cell lysate Lane7:The Brain tissue lysate of Mouse(40ug)
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BiowMW~ 90 kDa
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NoteFor research use only, not for use in diagnostic procedure.
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PropertiesIf you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translationanticorps
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Gene target
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Gene symbolRPS6KA5
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Short nameMSK1 (phospho-Thr581) polyclonal antibody
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TechniquePolyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit
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Alternative nameMSK1 (phosphorilated-Thr581) polyclonal (antibody to-)
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameribosomal protein S6 kinase A5
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Synonyms gene name
- ribosomal protein S6 kinase, 90kD, polypeptide 5
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-03-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MAPK activated protein kinases
- AGC family kinases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data