MLK3 (phospho-T277/S281) polyclonal antibody

• Catalog number
  BS64014-50ul
• Price
  Please ask
• Size
  50ul

• Alternative Name
  Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase; MAP3K11; MLK3; PTK1; SPRK
• Immunogen
  Synthetic phosphopeptide derived from human MLK3 around the phosphorylation site of Threonine 277 and Serine281.
• Swiss Prot
  Q16584
• Reactivity
  Human,Mouse,Rat
• Applications
  WB
• Application_all
  WB: 1:500~1:1000
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  p-MLK3 (T277/S281) polyclonal antibody detects endogenous levels of human MLK3 when phosphorylated at Thr602 and Ser281.
• Usage
  Western blot (WB) analysis of p-MLK3 (T277/S281) polyclonal antibody at 1:500 dilution Lane1:HEK293Twhole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:PC12 whole cell lysate
• BiowMW
  ~ 93, 100 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  MLK3   phospho-T277/S281  
• Gene symbol
  MAP3K11
• Short name
  MLK3 (phospho-T277/S281) polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit
• Alternative name
  MLK3 (phosphorilated-T277/S281) polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:6850
• Gene
  MAP3K11
• Long gene name
  mitogen-activated protein kinase kinase kinase 11
• Synonyms gene
  • MLK3
  • PTK1
• Synonyms
  • SPRK
  • MEKK11
• Locus
  11q13.1
• Discovery year
  1992-10-02
• Entrez gene record
  4296
• Pubmed identfication
  • 9267807
  • 8183572
• Classification
  • Mitogen-activated protein kinase kinase kinases
• VEGA ID
  OTTHUMG00000166529

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products