Caspase 5 p20 (D121) polyclonal antibody
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Catalog number
BS7048-100ul
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Price
Please ask
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Size
100ul
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Alternative Name
Caspase-5; CASP-5; ICE(rel)-III; Protease ICH-3; Protease TY; Caspase-5 subunit p20; CASP5; ICH3; Caspase5
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Immunogen
Synthetic peptide, corresponding to amino acids 100-150 of Human Caspase-5.
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Swiss Prot
P51878
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Reactivity
Human
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Applications
WB
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Application_all
WB: 1:500~1:1000
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Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
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Storage Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Specificity
Caspase-5 p20 (D121) polyclonal antibody detects endogenous levels of the large subunit of active caspase-5 resulting from cleavage at Asp121.
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Usage
Western blot (WB) analysis of Caspase-5 p20 (D121) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate(40ug) Lane3:HEK293T treated with UV for 5 minutes then repair for 24 hours whole cell lysate(40ug)
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BiowMW
~ 20, 48 kDa
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Note
For research use only, not for use in diagnostic procedure.
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Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Short name
Caspase 5 p20 (D121) polyclonal antibody
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Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit
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Alternative name
caspase 5 p20 (D121) polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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