CA II monoclonal antibody

• Catalog number
  MB9113-100ul
• Price
  Please ask
• Size
  100ul

• Alternative Name
  CAC, CA-II, CA2, can, CAN_ECOLI, Carbonate dehydratase 2, Carbonic anhydrase 2 antibody
• Immunogen
  Recombinant protein within Human Carbonic anhydrase 2 aa 50-220.
• Swiss Prot
  P00918 Human;P00920 Mouse;P27139 Rat
• Reactivity
  Human, Mouse, Rat
• Applications
  WB ICC IHC
• Application_all
  WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200
• Product
  Mouse IgG2a, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  Protein affinity purified.
• Storage Stability
  Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• Specificity
  CA II monoclonal antibody detects endogenous levels of CA II protein.
• Usage
  Western blot analysis of Carbonic anhydrase 2 on different cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:1,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: THP-1 cell lysateLane 2: HL-60 cell lysate
• BiowMW
  29 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  monoclonal  
• Short name
  CA II monoclonal antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse
• Alternative name
  CA II monoclonal (antibody to-)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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