Beclin 1 polyclonal antibody
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Catalog number
AP6020-50ul
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Price
Please ask
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Size
50ul
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Alternative Name
APG6; ATG 6; ATG6; ATG6 autophagy related 6 homolog; Bcl-2-interacting protein beclin; Beclin 1 (coiled coil moesin like BCL2 interacting protein); Beclin 1 autophagy related; Beclin-1; Beclin1; BECN 1; BECN1; BECN1; Coiled coil myosin like BCL2 interacting protein; Coiled-coil myosin-like BCL2-interacting protein; GT197; Protein GT197 antibod
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Immunogen
Recombinant fragment corresponding to a region within amino acids 141-451 of Human Beclin 1.
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Swiss Prot
Q14457
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Reactivity
Human,Mouse,Rat
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Applications
WB IHC
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Application_all
WB: 1:500~1:2000 IHC: 1:50~1:200
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Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
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Storage Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Specificity
Beclin 1 polyclonal antibody detects endogenous levels of Beclin 1 protein.
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Usage
Western blot (WB) analysis of Beclin 1 pAb at 1:2000 dilution Lane1:AML-12 whole cell lysate(40ug) Lane2:PMVEC whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:A549 whole cell lysate(40ug)
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BiowMW
~ 55 kDa
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Note
For research use only, not for use in diagnostic procedure.
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Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
BECN2, BECN1, BECN1P2
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Short name
Beclin 1 polyclonal antibody
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Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit
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Alternative name
Beclin 1 polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene name
beclin 2
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Synonyms gene
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Synonyms gene name
- beclin 1, autophagy related, pseudogene 1
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Locus
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Discovery year
2010-06-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
beclin 1
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Synonyms gene name
- beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
- beclin 1, autophagy related
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1998-11-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PIK3C3 complex subunits
- MicroRNA protein coding host genes
- Autophagy related
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
beclin 1 pseudogene 2
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Synonyms gene name
- beclin 1, autophagy related, pseudogene 2
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Locus
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Discovery year
2014-10-03
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Entrez gene record
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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