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Alternative Name
PKB, PKB alpha, AKT1, AKT 1, AKT, AKT1, AKT1, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein Kinase B Alpha, Protein kinase B, Proto-oncogene c-Akt, RAC Alpha, RAC, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha,
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Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser124 of human AKT1.
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Swiss Prot
P31749(Human) P31750(Mouse) P47196(Rat)
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Reactivity
Human, Mouse, Rat
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Applications
WB ICC/IF IHC IP
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Application_all
WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200
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Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification Purity
ProA affinity purified
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Storage Stability
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Specificity
AKT1 (Phospho-S124) polyclonal antibody detects endogenous levels of AKT1 protein only when phosphorylated at S124.
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Usage
ICC staining Phospho-AKT1(S124) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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BiowMW
56 kDa
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Note
For research use only, not for use in diagnostic procedure.
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Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
AKT1
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Short name
AKT1 (Phospho-S124) polyclonal antibody
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Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit
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Alternative name
v-akt murine thymoma viral oncogene homolog 1 (phosphorilated-S124) polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
v-akt murine thymoma viral oncogene homolog 1, AKT and CWS6 and PKB and PKB-ALPHA and PRKBA and RAC and RAC-ALPHA, AKT1 and IDBG-22709 and ENSG00000142208 and 207, phosphatidylinositol-3, nuclei, Akt1 and IDBG-174623 and ENSMUSG00000001729 and 11651, AKT1 and IDBG-635767 and ENSBTAG00000017636 and
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