AKT1 Mouse monoclonal antibody
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Catalog numberMB9025-100ul
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Price:
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Size100ul
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Alternative NameRAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC; PKBα;
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ImmunogenThis antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to a region of AKT1.
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Swiss ProtP31749
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ReactivityHuman, mouse
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ApplicationsWB ICC IHC FC
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Application_allWB: 1:2000 IHC/ICC: 1:100-1:200 FC: 100-1:200
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ProductMouse IgG2b, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification PurityProtein A affinity purified
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Storage StabilityStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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SpecificityThis antibody detects endogenous levels of AKT1 and does not cross-react with related proteins.
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UsageWestern blot (WB) analysis of AKT1 Mouse mAb at 1:2000 dilution Lane1:HepG2 whole cell lysate Lane2:MCF7 whole cell lysate Lane3:Hela whole cell lysate
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BiowMW~ 60 kDa
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NoteFor research use only, not for use in diagnostic procedure.
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PropertiesIf you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolAKT1
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Short nameAKT1 Mouse monoclonal antibody
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TechniqueMouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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HostMouse, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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SpeciesMouse, Mouses
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Alternative namev-akt murine thymoma viral oncogene homolog 1 Mouse monoclonal (antibody to-)
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Alternative techniqueantibodies, murine
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Alternative to gene targetv-akt murine thymoma viral oncogene homolog 1, AKT and CWS6 and PKB and PKB-ALPHA and PRKBA and RAC and RAC-ALPHA, AKT1 and IDBG-22709 and ENSG00000142208 and 207, phosphatidylinositol-3, nuclei, Akt1 and IDBG-174623 and ENSMUSG00000001729 and 11651, AKT1 and IDBG-635767 and ENSBTAG00000017636 and
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Gene info
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Identity
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Gene
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Long gene nameAKT serine/threonine kinase 1
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Synonyms gene name
- v-akt murine thymoma viral oncogene homolog 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Pleckstrin homology domain containing
- AKT kinases
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data