USP14 Antibody

  • Catalog number
    6134-50
  • Price
    209.09 USD
  • Size
    50 μg
  • Antibody Target
    USP14
  • Target Alternative Name
    Deubiquitinating enzyme 14, TGT, Ubiquitin carboxyl-terminal hydrolase 14, Ubiquitin-specific processing protease 14, Ubiquitin thioesterase 14, Ubiquitin-specific-processing protease 14
  • Antibody Type
    Polyclonal
  • Species Reactivities
    Human
  • Immunogen Sequence
    Recombinant full length protein
  • Accession
    P54578
  • Gene Id
    9097
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    50 µg of antibody in PBS containing 10% glycerol
  • Purification
    Purified from egg yolk
  • Application
    Western blot
  • Application And Usages
    Western blot: Robust detection of 100 ng of recombinant protein was possible when antibody was used at a final concentration of 5 μg/mL
  • Description
    Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. In humans, there are three proteasomal DUBs: PSMD14 (POH1/RPN11), UCH37 (UCH-L5), and Ubiquitin-Specific Protease 14, which is also known as the 60 kDa subunit of tRNA-guanine transglycosylase (USP14/TGT60 kDa). USP14 is recruited to the proteasome through its reversible association with the PSMD2 (S2/hRPN1) subunit of the 19S regulatory particle. Whereas PSMD14 appears to promote substrate degradation, USP14 is thought to antagonize substrate degradation. While the underlying mechanism for the opposing roles of these two proteasomal DUBs is still uncertain, it is thought that USP14 removes ubiquitin from substrate upon docking of the substrate with the 26S proteasome. Furthermore, USP14 trims ubiquitin residues from the distal end of the polyubiquitin chain, thus decreasing the affinity of the chain for the ubiquitin receptors of the proteasome, and allowing for enhanced substrate stability. Studies have elucidated a physiologic role for USP14 in regulating synaptic activity in mammals. Research studies have shown that targeting this activity with small molecule inhibitors has potential benefits for the treatment of neurodegenerative diseases and cancer.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    USP14  
  • Gene symbol
    USP14
  • Short name
    USP14 Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Chicken IgG
  • Alternative name
    ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) (antibody to-)
  • Alternative technique
    antibodies
  • Alternative to gene target
    ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase), TGT, USP14 and IDBG-259 and ENSG00000101557 and 9097, proteasome binding, Cell surfaces, Usp14 and IDBG-128322 and ENSMUSG00000047879 and 59025, BT.20599 and IDBG-644085 and ENSBTAG00000019214 and 505106
Gene info
  • Identity
  • Gene
  • Long gene name
    ubiquitin specific peptidase 14
  • Synonyms gene name
    • ubiquitin specific protease 14 (tRNA-guanine transglycosylase)
    • ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1999-02-01
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Ubiquitin specific peptidases
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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