ToxOut™ Phase Extraction Endotoxin Removal Kit

• Catalog number
  K2507-100
• Price
  Please ask
• Size
  10 Columns

• Description
  Quickly and effectively eliminate endotoxins to < 0.05 EU/ml in solutions containing proteins or pharmacologically important components
• Applications
  Ideal for processing small scale solution samples (0.1 ml - 0.5 ml),Effectively eliminate endotoxins to < 0.05 EU/ml
• Features Benefits
  Final products are suitable for potential downstream therapeutic applications as ligand and buffers are nontoxic,Simple and fast procedure: Takes less than one hour to get endotoxin-free sample,High Capacity: up to 99.99% endotoxin can be removed in a single extraction at 1E+7 EU/ml,One kit can clean up to 5 ml protein sample at 1-5 mg/ml of concentration,Lower endotoxin level to < 0.05 EU/ml in a single extraction from up to 5,000 EU/ml sample,Very low residual detergent: < 1 µg/ml,High Sample Recovery: >90% recovery with protein solution samples (typically > 95%)
• Storage Conditions
  4ºC
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Endotoxin is a lipopolysaccharide (LPS) complex located in the outer membrane of gram-negative bacteria. A single E.coli bacterium contains ~2 million LPS molecules. During experimental procedures, large amount of endotoxin is shed and can easily contaminate labware, buffers and downstream products. In vitro, endotoxin causes a variety of problems in cell-based research. In vivo, endotoxin may cause various side effects, including inflammatory response, organ failure or septic shock in host organisms. Therefore, it is critical to remove endotoxin from samples and products. BioVision‘s ToxOutTM Phase Extraction Endotoxin Removal Kit can effectively eliminate high concentration endotoxin in solutions containing proteins or pharmacologically important components down to 90%. BioVision‘s ToxOutTM Phase Extraction Endotoxin Removal Kit is based on Triton X-114 Phase Extraction (PE), which shows strong endotoxin removal capabilities. The residual Triton X-114 is further eliminated with our proprietary beads to achieve minimal detergent level in the final samples. The protein recovery is >90% depending on individual protein and typically greater than 95%.
• Data Sheet Link

• Gene target
  ToxOut™   Phase   Extraction   Endotoxin   Removal   Kit  
• Short name
  ToxOut™ Phase Extraction Endotoxin Removal Kit
• Alternative name
  ToxOut™ Phase Extraction Endotoxin Removal reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Limulus Test
• Concept
  Scope note: Sensitive method for detection of bacterial endotoxins and endotoxin-like substances that depends on the in vitro gelation of Limulus amebocyte lysate (LAL), prepared from the circulating blood (amebocytes) of the horseshoe crab, by the endotoxin or related compound. Used for detection of endotoxin in body fluids and parenteral pharmaceuticals.
• Tree numbers
  • E01.370.225.124.470
  • E01.370.225.875.150.570
  • E05.091.570
  • E05.200.124.470
  • E05.200.875.150.570
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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