Threonine Dehydrogenase, Recombinant

• Catalog number
  P1256-150
• Price
  Please ask
• Size
  150 ug

• Synonyms
  ThrDH
• Alternative_names
  ThrDH
• Recombinant
  Yes
• Source
  E.coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measure by its ability to converts 1 umole of L-threonine into 2-amino-3-oxobutyrate, with concomitant reduction of β-NAD+ to NADH, per minute at 25°C and pH 8.5.
• Biological activity
  Specific activity is ≥ 2 U/mg
• Results
  ≥ 2 U/mg
• Molecular Weight
  36.8 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Proprietary buffer
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in distilled water.
• Background Information
  Threonine dehydrogenase (EC 1.1.1.103) is a β-NAD+ coenzyme dependent enzyme, from the group of short chain alcohol dehydrogenases. It is a key enzyme in L threonine catabolism in microorganisms and mammals. The enzyme catalyzes conversion of l-threonine to 2-amino-3 ketobutyrate, with simultaneous reduction of NAD+.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Threonine   Dehydrogenase  
• Gene symbol
  TDH
• Short name
  Threonine Dehydrogenase, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  Threonine Dehydrogenase, Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:15547
• Gene
  TDH
• Long gene name
  L-threonine dehydrogenase (pseudogene)
• Synonyms gene name
  • L-threonine dehydrogenase
• Synonyms
  • FLJ25033
  • SDR14E1P
• Synonyms name
  • short chain dehydrogenase/reductase family 14E, member 1 (pseudogene)
• GenBank acession
  • AJ301562
• Locus
  8p23.1
• Discovery year
  2001-04-25
• Entrez gene record
  157739
• Pubmed identfication
  • 11896452
  • 12361482
  • 19027726
• RefSeq identity
  • NR_001578
• Classification
  • Short chain dehydrogenase/reductase superfamily
• VEGA ID
  OTTHUMG00000165365

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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