Thioredoxin Reductase 2 Antibody (7B2)
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Catalog number6165-100
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PricePlease ask
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Size100 μl
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Antibody TargetThioredoxin Reductase 2
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Target Alternative NameThioredoxin Reductase, GRIM-12, mgC9145, TR, TRXR2.
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Antibody TypeMonoclonal
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Clone7B2
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Species ReactivitiesHuman
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Immunogen SequenceRecombinant human protein purified from E.coli
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AccessionQ9NNW7
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Gene Id10587
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AppearanceColorless liquid
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FormLiquid
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Concentration1 mg/ml
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Formulation100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
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PurificationAmmonium sulphate precipitation purified antibody
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ApplicationWestern blot,IP
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Positive ControlWB analysis of cell lysates
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Application And UsagesWestern blot: 1:1000, IP: 1-2 µl.
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DescriptionThe mammalian thioredoxin reductases (TrxRs) are a family of seleno-cysteine containing pyridine nucleotide-disulfide oxido-reductases. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate seleno-cysteine (SeCys) residue in the conserved sequence(-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55 – 58 kDa in molecular size and composed of three isoforms including cytosolic TrxR1, mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various biological phenomena. TrxR2 maintains thioredoxin in a reduced state. Implicated in the defenses against oxidative stress. May play a role in redox-regulated cell signaling.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolSCG5
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Short nameThioredoxin Reductase 2 Antibody (7B2)
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeMouse IgG 1
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Alternative nameThioredoxin Reductase 2 (antibody to-) (7B2)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namesecretogranin V
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Synonyms gene
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Synonyms gene name
- secretory granule, neuroendocrine protein 1 (7B2 protein)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-09-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Granins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data