TECK (CCL25), human recombinant
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Catalog number
7214-10
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Price
Please ask
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Size
10 ug
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Synonyms
Thymus Expressed Chemokine, CCL25
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Alternative_names
Thymus Expressed Chemokine, CCL25
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Description
Chemotactic towards activated macrophages, thymocytes and dendritic cells
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Recombinant
Yes
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Source
E. Coli
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Purity by SDS PAGE
≥98%
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Assay
SDS-PAGE
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Endotoxin Level
< 0.1 ng/ug of protein (
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Activity Specifications test method
Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.
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Biological activity
Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.
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Molecular Weight
14.2 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Acetic acid.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Background Information
TECK is a CC chemokine, specifically expressed by thymic stromal cells, and signals through the CCR9 receptor. TECK is chemotactic towards activated macrophages, thymocytes and dendritic cells. Recombinant human TECK is a 14.2 kDa protein containing 127 amino acid residues, including the four conserved cysteine residues present in CC chemokines.
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Amino acid sequence
QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSRKNVSLL ISANSGL
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CCL25
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Short name
TECK (CCL25), recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
TECK (chemokine (C-C motif) ligand 25), H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
chemokine (C-C motif) ligand 25, Ckb15 and SCYA25 and TECK, CCL25 and IDBG-24184 and ENSG00000131142 and 6370, chemokine receptor binding, Extracellular, Ccl25 and IDBG-131488 and ENSMUSG00000023235 and 20300, CCL25 and IDBG-643756 and ENSBTAG00000005581 and 615986
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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