Sulfo-NHS-SS-Biotin
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Catalog number2323-100
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PricePlease ask
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Size100 mg
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Alternative_nameSulfosuccinimidyl-2-(biotinamido)-ethyl-1,3'-dithiopropionate; Biotin disulfide N-Hydroxysulfosuccinimide ester
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DescriptionKEY FEATURES: • Sulfo-NHS-SS-Biotin is amine-reactive and • Has a long chain spacer arm (24.3 Å) separating the biotin group from the amino-reactive sulfo-NHS ester • Water-soluble; can be used under physiologic conditions • It is possible to cleave the biotin group from the cross-linked target protein under reducing conditions, such as in the presence of BME, DTT or TCEP APPLICATIONS: • Sulfo-NHS-SS-Biotin is ideal for biotinylating antibody or protein that can then be used for protein purification, detection or immobilization • Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent
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CAS Number325143-98-4
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Structure AvailableYes
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Salt FormNo
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Molecular FormulaC₁₉H₂₇O₉N₄S₄Na
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Molecular Weight606.69
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Cell PermeableNo
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Purity≥80% by NMR
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SolubilitiesH₂O (~ 6 mg/ml)
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HandlingProtect from moisture
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Tag LineA water-soluble, dislulfide-reducible, protein biotinylation reagent.
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Storage Condition+4 ̊C
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Shipping ConditionGel Pack
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Shelf Life36 months
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MDL NumberMFCD00078533
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SMILESO=C(NCCSSCCC(ON1C(C(S(=O)(O[Na])=O)CC1)=O)=O)CCCCC2SCC(C2N3)NC3=O
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InChiInChI=1S/C19H30N4O8S4.Na/c24-15(4-2-1-3-13-17-12(11-32-13)21-19(27)22-17)20-7-10-34-33-9-6-16(25)31-23-8-5-14(18(23)26)35(28,29)30;/h12-14,17H,1-11H2,(H,20,24)(H2,21,22,27)(H,28,29,30);/q;+1/p-1
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InChi KeyHBLXCNOBPVOLAK-UHFFFAOYSA-M
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MSDS AvailableYes
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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Gene target
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Gene symbolNHS, NHS-AS1, TRIM68, GYPB, SHOX
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Short nameSulfo-NHS-SS-
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LabelBiotin
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Alternative nameSulfo-NHS-SS-biotinilated
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Gene info
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Identity
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Gene
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Long gene nameNHS actin remodeling regulator
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Synonyms gene name
- Nance-Horan syndrome (congenital cataracts and dental anomalies)
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Locus
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Discovery year1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameNHS antisense RNA 1
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Synonyms gene name
- NHS antisense RNA 1 (non-protein coding)
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Locus
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Discovery year2011-07-29
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametripartite motif containing 68
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Synonyms gene
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Synonyms gene name
- ring finger protein 137
- tripartite motif-containing 68
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-05-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ring finger proteins
- Tripartite motif containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameglycophorin B (MNS blood group)
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Synonyms gene
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Synonyms gene name
- glycophorin B (includes Ss blood group)
- glycophorin B (Ss blood group)
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Synonyms
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Locus
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Discovery year1988-08-31
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Entrez gene record
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RefSeq identity
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Classification
- Blood group antigens
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameshort stature homeobox
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-06-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Pseudoautosomal region 1
- PRD class homeoboxes and pseudogenes
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VEGA ID
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Locus Specific Databases