Sulfo-NHS-LC-Biotin
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Catalog number2326-50
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Price205.59 USD
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Size50 mg
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Alternative_nameSulfosuccinimidyl 6-(biotinamido) Hexanoate; Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt
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DescriptionKEY FEATURES: • Amine-reactive; has a spacer with arm length of 22.4 Å which helps to minimize steric hindrance to biotin labeling • More water-soluble than the corresponding NHS compound without the sulfo group • Membrane-impermeable • Forms permanent amide bonds and the spacer arm cannot be cleaved APPLICATIONS: • Sulfo-NHS-LC-Biotin is ideal for biotinylating antibody or protein that can then be used for protein detection, purification or immobilization • Sulfo-NHS-LC-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent
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CAS Number127062-22-0
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Structure AvailableYes
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Salt FormNo
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Molecular FormulaC₁₄H₁₈O₈N₃S₂Na
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Molecular Weight556.59
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Cell PermeableNo
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Purity≥90% by NMR
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SolubilitiesH₂O (~ 20 mg/ml)
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HandlingProtect from moisture
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Tag LineSulfo-NHS-LC-Biotin is a water-soluble protein biotinylation reagent.
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Storage Condition-20 ̊C
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Shipping ConditionGel Pack
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Shelf Life36 months
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MDL NumberMFCD00078534
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PubChem CID16219001
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SMILESO=C([MH]1)NC2C1CSC2CCCCC(NCCCCCC(ON3C(C(S(=O)(O[Na])=O)CC3=O)=O)=O)=O
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InChiInChI=1S/C20H30N4O9S2.Na/c25-15(7-4-3-6-13-18-12(11-34-13)22-20(29)23-18)21-9-5-1-2-8-17(27)33-24-16(26)10-14(19(24)28)35(30,31)32;/h12-14,18H,1-11H2,(H,21,25)(H2,22,23,29)(H,30,31,32);
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InChi KeyRDSJVNGBJUJRRW-UHFFFAOYSA-N
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MSDS AvailableYes
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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Gene target
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Gene symbolNHS, NHS-AS1, PTPN7, CT83
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Short nameSulfo-NHS-LC-
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LabelNHS-LC-Biotin
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Alternative nameSulfo-NHS-LC-biotinilated
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Gene info
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Identity
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Gene
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Long gene nameNHS actin remodeling regulator
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Synonyms gene name
- Nance-Horan syndrome (congenital cataracts and dental anomalies)
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Locus
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Discovery year1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameNHS antisense RNA 1
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Synonyms gene name
- NHS antisense RNA 1 (non-protein coding)
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Locus
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Discovery year2011-07-29
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase non-receptor type 7
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-03-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein tyrosine phosphatases non-receptor type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecancer/testis antigen 83
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Synonyms gene
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Synonyms gene name
- chromosome X open reading frame 61
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2007-06-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID