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Alternative_name
N-succinimidyl-3-(2-pyridyldithio)propionate; 3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester; 3-(2-Pyridyldithio)propionic acid NHS ester
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Description
KEY FEATURES: • SPDP contains a dithiopyridyl group which is reactive towards cysteines (-SH groups) and an NHS group, which is useful when targeting primary amines (-NH2 groups) • SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343 nm, the reaction can be easily followed • Contains a disulfide linkage in its spacer arm with a length of 6.8 Å • The spacer is cleavable with reducing agent, such as DTT, TCEP, etc. APPLICATIONS: • Reversible crosslinking of proteins • N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains • Useful for adding sulfhydryl groups to proteins by attachment to lysine residues • Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates
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CAS Number
68181-17-9
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Structure Available
Yes
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Salt Form
No
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Molecular Formula
C₁₂H₁₂N₂O₁₀
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Molecular Weight
312.36
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Cell Permeable
No
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Purity
≥90% by NMR
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Solubilities
DMSO or DMF
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Handling
Protect from moisture
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Tag Line
A cleavable heterobifunctional crosslinker.
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Storage Condition
-20 ̊C
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Shipping Condition
Gel Pack
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Shelf Life
36 months
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MDL Number
MFCD00009636
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PubChem CID
100682
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SMILES
C1CC(=O)N(C1=O)OC(=O)CCSSC2=CC=CC=N2
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InChi
InChI=1S/C12H12N2O4S2/c15-10-4-5-11(16)14(10)18-12(17)6-8-19-20-9-3-1-2-7-13-9/h1-3,7H,4-6,8H2
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InChi Key
JWDFQMWEFLOOED-UHFFFAOYSA-N
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MSDS Available
Yes
