SIRT2 Inhibitor Screening Assay Kit (Fluorometric)
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Catalog number
K322-100
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Price
Please ask
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Size
100 assays
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Description
HTS, includes inhibitor
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Summary
• Detection method- Fluorescence (Ex/Em 395/541 nm) • Species reactivity- Mammalian • Application- • Screening and characterization of Sirtuin inhibitors.
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Sample Type
N/A
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Features Benefits
• Simple procedure • Fast and convenient • The assay is sensitive, reliable, and high-throughput adaptable.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Sirtuin or Sir2 proteins are a class of proteins that possess either histone deacetylase or mono-ribosyltransferase activity. Sirtuins have been implicated in influencing aging and regulating transcription, apoptosis and stress resistance, as well as energy efficiency and alertness during low-calorie situations. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction couples lysine deacetylation to NAD hydrolysis. This hydrolysis yields O-acetyl-ADP-ribose, the deacetylated substrate and nicotinamide, itself an inhibitor of sirtuin activity. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. In BioVision’s Sirtuin inhibitor screening Kit, Sirtuin deacetylates the substrate and then the developer cleaves the deacetylated substrate to release the fluorescent group, which can be detected fluorometrically at Ex/Em = 395/541 nm. In the presence of SIRT inhibitor, the deacetylation will be impeded which prevents the cleavage of the substrate to release the fluorescent group. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high-throughput screening of SIRT2 inhibitors. Inhibitor control (Nicotinamide) is included to compare the efficacy of the test inhibitors.
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Test
Biovision supplies other types of Assays as 1.
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Additional description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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Gene target
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Gene symbol
SIRT2
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Short name
SIRT2 Inhibitor Screening Assay Kit (Fluorometric)
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Technique
Assay, assays
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Host
Assay
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Alternative name
SIRT2 suppressor detection test reagent (fluorescence)
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
sirtuin 2
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Synonyms gene
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Synonyms gene name
- sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 2
- sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
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GenBank acession
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Locus
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Discovery year
1998-10-14
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
Product images
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