Reactive Oxygen Species (ROS) Detection Assay Kit

• Catalog number
  K936-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly Sensitive Assay that measures Reactive Oxygen Species. Non-radioactive, High-Throughput
• Summary
  • Detection method- Fluorescence (Ex/Em = 495/529 nm). • Applications-• Measurement of intracellular levels of ROS • Screening compounds with a stress inducing and/or antioxidant function.
• Detection Method
  Fluorescence (Ex/Em = 495/529 nm).
• Species Reactivity
  Eukaryotes
• Applications
  • Measurement of intracellular levels of ROS. • Screening compounds with a stress inducing and/or antioxidant function
• Sample Type
  Suspension or adherent cell cultures
• Features Benefits
  • Simple & Rapid Protocol • Convenient: Non-Radioactive, no special handling or disposal required • High-Throughput • Accurate: reproducible results with low intra & inter assay variability
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Constant generation of low levels of reactive oxygen species (ROS) and free radicals is a basic feature of all living cells. Low levels of ROS play an essential role in signaling pathways, whereas increased under oxidative stress, ROS activity result in damage to nucleic acids, proteins and membrane lipids. Accumulation of ROS during oxidative stress is also associated with aging, apoptosis or necrosis, and is implicated in pathological conditions such as; vascular diseases, diabetes, renal ischemia, arteriosclerosis, pulmonary disorders, inflammatory diseases, and cancer. Cellular activity of ROS is offset by antioxidants, numerous repair systems, and replacement of damaged DNA. Probes for measuring intracellular ROS levels provide important tools to study oxidative stress inducers and effects of antioxidant therapies. BioVision’s ROS Detection Assay Kit is designed for detection of hydroxyl, peroxyl, or other reactive oxygen species in live cells. We utilize H2DCFDA, a unique cell-permeable fluorogenic probe, compatible with phenol red, FBS and BSA to detect reactive oxygen species in live cells. Upon the cell entry, H2DCFDA is modified by cellular esterases to form a non-fluorescent H2DCF. Oxidation of H2DCF by intracellular ROS yields highly a fluorescent product that can be detected by FACS, microplate reader, or fluorescence microscope (Ex/Em 495/529 nm). The fluorescence intensity is proportional to the ROS levels. Our kit provides a simple and specific assay for the real-time measurement of global levels of ROS in living cells. We include sufficient reagents to perform 100 assays and a common ROS inducer as a control for measurement of ROS levels or antioxidant activity with high sensitivity, specificity and accuracy
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol. Monoclonals are usually produced in mouse as host species and polyclonals in rabbit or goat. Multi species reactivity is achieved by selecting consensus epitopes when blasting the gene for a reactive, innugenic epitope that is the same in the different target species.

• Gene target
  Reactive   Oxygen   ROS   Kit  
• Gene symbol
  ROS1, GUCY2F, GUCY2D
• Short name
  Reactive Oxygen (ROS) Assay Kit
• Technique
  detection, Assay, assays
• Host
  Assay
• Alternative name
  Reactive Oxygen Species (ROS) quantification test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:10261
• Gene
  ROS1
• Long gene name
  ROS proto-oncogene 1, receptor tyrosine kinase
• Synonyms gene name
  • v-ros avian UR2 sarcoma virus oncogene homolog 1
  • v-ros UR2 sarcoma virus oncogene homolog 1 (avian)
  • c-ros oncogene 1 , receptor tyrosine kinase
• Synonyms
  • MCF3
  • ROS
  • c-ros-1
• GenBank acession
  • M13599
• Locus
  6q22.1
• Discovery year
  1986-01-01
• Entrez gene record
  6098
• Pubmed identfication
  • 1611909
• Classification
  • Receptor tyrosine kinases
  • Fibronectin type III domain containing
• VEGA ID
  OTTHUMG00000016188
• Locus Specific Databases
  • LRG_997

Gene info

• Identity
  HGNC:4691
• Gene
  GUCY2F
• Long gene name
  guanylate cyclase 2F, retinal
• Synonyms
  • GUC2DL
  • GC-F
  • RetGC-2
  • ROS-GC2
  • CYGF
• Synonyms name
  • guanylate cyclase 2D-like, membrane (retina-specific)
• GenBank acession
  • L37378
• Locus
  Xq22.3-q23
• Discovery year
  1997-12-05
• Entrez gene record
  2986
• Pubmed identfication
  • 8838319
  • 7777544
• RefSeq identity
  • NM_001522
• Classification
  • Guanylate cyclase receptors
• VEGA ID
  OTTHUMG00000022184
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:4689
• Gene
  GUCY2D
• Long gene name
  guanylate cyclase 2D, retinal
• Synonyms gene
  • CORD6
  • LCA
  • GUC2D
  • GUC1A4
• Synonyms gene name
  • cone rod dystrophy 6
  • guanylate cyclase 2D, membrane (retina-specific)
• Synonyms
  • retGC
  • RETGC-1
  • ROS-GC1
  • CYGD
  • LCA1
• Synonyms name
  • rod outer segment membrane guanylate cyclase
  • retinal guanylate cyclase 1
• GenBank acession
  • L26921
• Locus
  17p13.1
• Discovery year
  1993-11-09
• Entrez gene record
  3000
• Pubmed identfication
  • 1356371
  • 12552567
• RefSeq identity
  • NM_000180
• Classification
  • Guanylate cyclase receptors
• VEGA ID
  OTTHUMG00000108169
• Locus Specific Databases
  • Mutations of the Retinal Guanylate Cyclase Gene and the Retinal Guanylate Cyclase-activating Protein Gene

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