Rabex-5 Antibody

• Catalog number
  3905-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Rabex-5
• Target Alternative Name
  Rab5 GDP/GTP exchange factor
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Synthetic peptide at C-terminal (BV-M11)
• Accession
  Q9UJ41
• Gene Id
  27342
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) of antibody in PBS, 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol, pH 7.2
• Purification
  Affinity purified
• Application
  Western blot
• Positive Control
  Rat Kidney lysate, 3T3 cell lysate
• Application And Usages
  Western blot: 1:200
• Description
  Rabex-5, also called RabGEF1 and RAP1, was identified as a guanine nucleotide exchange factor (GEF) for Rab5, a member of the Ras superfamily of small Rab GTPases (1). Rabex-5 generates the GTP-bound active form of Rab5 and forms a tight association with its effector protein Rabaptin-5. This complex localizes to endosomal membranes where it functions as a key regulator of vesicular trafficking during early endocytosis. Rabex-5 is also monoubiquitinated and has ubiquitin ligase activity that regulates its recruitment to early endosomes. The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis. Through its control of endosomal trafficking and endocytosis, Rabex-5 has been shown to negatively regulate NGF-mediated neurite outgrowth as well as FcεRI-dependent mast cell activation.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Rabex-5  
• Gene symbol
  RABGEF1
• Short name
  Rabex-5 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Rabex-5 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:17676
• Gene
  RABGEF1
• Long gene name
  RAB guanine nucleotide exchange factor 1
• Synonyms gene name
  • RAB guanine nucleotide exchange factor (GEF) 1
• Synonyms
  • rabex-5
  • RABEX5
• GenBank acession
  • AJ250042
• Locus
  7q11.21
• Discovery year
  2003-07-14
• Entrez gene record
  27342
• Pubmed identfication
  • 12505986
  • 11098082
• RefSeq identity
  • NM_014504
• Classification
  • VPS9 domain containing
• VEGA ID
  OTTHUMG00000129547

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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